truC:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TruC

Synonyms

tRNA pseudouridine synthase[1], B2791[2][1], TruC[2][1], YqcB[2][1] , ECK2785, JW2762, yqcB, b2791

Product description

tRNA pseudouridine 65 synthase[2][3]

tRNA U65 pseudouridine synthase; modification found in tRNA(Ile1)[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006145

F

Seeded from EcoCyc (v14.0)

complete

GO:0006399

tRNA metabolic process

PMID:11720289[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006224

F

Seeded from EcoCyc (v14.0)

complete

GO:0009451

RNA modification

PMID:11720289[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020103

F

Seeded from EcoCyc (v14.0)

complete

GO:0031119

tRNA pseudouridine synthesis

PMID:11720289[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0009982

pseudouridine synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006145

F

Seeded from EcoCyc (v14.0)

complete

GO:0009982

pseudouridine synthase activity

PMID:11720289[5]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0009982

pseudouridine synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006224

F

Seeded from EcoCyc (v14.0)

complete

GO:0008033

tRNA processing

PMID:11720289[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0009982

pseudouridine synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020103

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MLEILYQDEW LVAVNKPSGW LVHRSWLDRD EKVVVMQTVR DQIGQHVFTA HRLDRPTSGV
LLMGLSSEAG RLLAQQFEQH QIQKRYHAIV RGWLMEEAVL DYPLVEELDK IADKFAREDK
GPQPAVTHYR GLATVEMPVA TGRYPTTRYG LVELEPKTGR KHQLRRHLAH LRHPIIGDSK
HGDLRQNRSG AEHFGLQRLM LHASQLSLTH PFTGEPLTIH AGLDDTWMQA LSQFGWRGLL
PENERVEFSA PSGQDGEISS
Length

260

Mol. Wt

29.665 kDa

pI

7.1 (calculated)

Extinction coefficient

41,940 (calc based on 6 Y, 6 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

10..170

PF00849 RNA pseudouridylate synthase

PMID:19920124[6]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=truC taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130698

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947242

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0009147

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AA41

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7449

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13171

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947242

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120004012

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2963

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 5.4 Del Campo, M et al. (2001) Identification and site of action of the remaining four putative pseudouridine synthases in Escherichia coli. RNA 7 1603-15 PubMed
  6. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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