tolB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TolB

Synonyms

periplasmic protein[1], B0740[2][1], Lky[2][1], LkyA[2][1], TolB[2][1] , ECK0729, JW5100, b0740

Product description

TolB[2][3];

Component of Tol-Pal Cell Envelope Complex[2]; The Tol-Pal Cell Envelope Complex[3]; The Colicin A Import System[3]; Colicin S4 Transport System[3]

Uptake of group A colicins, mutants are tolerant to colicins E1-3, A, and K[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0030288

outer membrane-bounded periplasmic space

PMID:2687247[5]

IDA: Inferred from Direct Assay

C

Figure 8

complete

GO:0005515

protein binding

PMID:9701827[6]

IPI: Inferred from Physical Interaction

EcoliWiki:pal


F

in vivo cross-linking

complete

GO:0005515

protein binding

PMID:9701827[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0043213

bacteriocin transport

PMID:9701827[6]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0017038

protein import

PMID:9701827[6]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0015031

protein transport

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007195

P

Seeded from EcoCyc (v14.0)

complete

GO:0015031

protein transport

PMID:9701827[6]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0015031

protein transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0653

P

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

PMID:7929011[7]

IDA: Inferred from Direct Assay

C

complete

GO:0017038

protein import

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00671

P

Seeded from EcoCyc (v14.0)

complete

GO:0030288

outer membrane-bounded periplasmic space

PMID:7929011[7]

IDA: Inferred from Direct Assay

C

complete

GO:0017038

protein import

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014167

P

Seeded from EcoCyc (v14.0)

complete

GO:0043213

bacteriocin transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0080

P

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:9701827[6]

IPI: Inferred from Physical Interaction

EcoliWiki:lpp


F

in vivo cross-linking

complete

GO:0005515

protein binding

PMID:9701827[6]

IPI: Inferred from Physical Interaction

EcoliWiki:ompA


F

in vivo cross-linking

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of Tol-Pal Cell Envelope Complex

could be indirect

Protein

rbsB

PMID:19402753[8]

MALDI(Z-score):39.052235

Protein

Subunits of The Tol-Pal Cell Envelope Complex

could be indirect

Protein

Subunits of The Colicin A Import System

could be indirect

Protein

Subunits of Colicin S4 Transport System

could be indirect

Protein

Pal

PMID:9701827[6]

in vivo cross-linking

Protein

Lpp

PMID:9701827[6]

in vivo cross-linking

Protein

OmpA

PMID:9701827[6]

in vivo cross-linking

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

periplasm

From EcoCyc[3]

Periplasm

PMID:17233825[9], PMID:7929011[7]

EchoLocation:tolB


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKQALRVAFG FLILWASVLH AEVRIVIDSG VDSGRPIGVV PFQWAGPGAA PEDIGGIVAA
DLRNSGKFNP LDRARLPQQP GSAQEVQPAA WSALGIDAVV VGQVTPNPDG SYNVAYQLVD
TGGAPGTVLA QNSYKVNKQW LRYAGHTASD EVFEKLTGIK GAFRTRIAYV VQTNGGQFPY
ELRVSDYDGY NQFVVHRSPQ PLMSPAWSPD GSKLAYVTFE SGRSALVIQT LANGAVRQVA
SFPRHNGAPA FSPDGSKLAF ALSKTGSLNL YVMDLASGQI RQVTDGRSNN TEPTWFPDSQ
NLAFTSDQAG RPQVYKVNIN GGAPQRITWE GSQNQDADVS SDGKFMVMVS SNGGQQHIAK
QDLATGGVQV LSSTFLDETP SLAPNGTMVI YSSSQGMGSV LNLVSTDGRF KARLPATDGQ
VKFPAWSPYL
Length

430

Mol. Wt

45.954 kDa

pI

7.5 (calculated)

Extinction coefficient

63,370 (calc based on 13 Y, 8 W, and 0 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-21

UniProt Manual:Signal Peptides

UniProt:P0A855

Domain

8..126

PF04052 TolB amino-terminal domain

PMID:19920124[10]

Domain

197..226

PF07676 WD40-like Beta Propeller Repeat

PMID:19920124[10]

Domain

236..272

PF07676 WD40-like Beta Propeller Repeat

PMID:19920124[10]

Domain

280..316

PF07676 WD40-like Beta Propeller Repeat

PMID:19920124[10]

Domain

367..402

PF07676 WD40-like Beta Propeller Repeat

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tolB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128715

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945429

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002517

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A855

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11008

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11008

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945429

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000997

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1001

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Levengood, SK & Webster, RE (1989) Nucleotide sequences of the tolA and tolB genes and localization of their products, components of a multistep translocation system in Escherichia coli. J. Bacteriol. 171 6600-9 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 Clavel, T et al. (1998) TolB protein of Escherichia coli K-12 interacts with the outer membrane peptidoglycan-associated proteins Pal, Lpp and OmpA. Mol. Microbiol. 29 359-67 PubMed
  7. ↑ 7.0 7.1 7.2 Isnard, M et al. (1994) Maturation and localization of the TolB protein required for colicin import. J. Bacteriol. 176 6392-6 PubMed
  8. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  9. ↑ Gerding, MA et al. (2007) The trans-envelope Tol-Pal complex is part of the cell division machinery and required for proper outer-membrane invagination during cell constriction in E. coli. Mol. Microbiol. 63 1008-25 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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