tatD:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

TatD

Synonyms

DNase, magnesium-dependent[1], YigX[2][1], YigW[2][1], MttC[2][1], TatD[2][1], B4483[2][1] , ECK3833, JW5931, mttC, yigW, yigX, b4483

Product description

magnesium-dependent DNase[2][3]

Mg-dependent cytoplasmic DNase; last gene in tatA operon, mutant has no Tat phenotype[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:10747959[5]

IDA: Inferred from Direct Assay

C

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

PMID:10747959[5]

IDA: Inferred from Direct Assay

F

Ni2+ and Zn2+ ion binding

complete

GO:0016888

endodeoxyribonuclease activity, producing 5'-phosphomonoesters

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001130

F

Seeded from EcoCyc (v14.0)

complete

GO:0004536

deoxyribonuclease activity

PMID:10747959[5]

IDA: Inferred from Direct Assay

F

in the presence of Mg2+, but not Ca2+ or Zn2+

complete

GO:0016888

endodeoxyribonuclease activity, producing 5'-phosphomonoesters

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR015992

F

Seeded from EcoCyc (v14.0)

complete

GO:0016888

endodeoxyribonuclease activity, producing 5'-phosphomonoesters

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018228

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

Cytosol

subcellular fractionation

PMID:10747959[5]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MFDIGVNLTS SQFAKDRDDV VACAFDAGVN GLLITGTNLR ESQQAQKLAR QYSSCWSTAG
VHPHDSSQWQ AATEEAIIEL AAQPEVVAIG ECGLDFNRNF STPEEQERAF VAQLRIAADL
NMPVFMHCRD AHERFMTLLE PWLDKLPGAV LHCFTGTREE MQACVAHGIY IGITGWVCDE
RRGLELRELL PLIPAEKLLI ETDAPYLLPR DLTPKPSSRR NEPAHLPHIL QRIAHWRGED
AAWLAATTDA NVKTLFGIAF
Length

260

Mol. Wt

28.973 kDa

pI

5.1 (calculated)

Extinction coefficient

37,470 - 38,345 (calc based on 3 Y, 6 W, and 7 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

2..257

PF01026 TatD related DNase

PMID:19920124[6]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=tatD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:49176429

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:2847752

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0174116

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P27859

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11481

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11481

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:2847752

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001439

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1446

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 Wexler, M et al. (2000) TatD is a cytoplasmic protein with DNase activity. No requirement for TatD family proteins in sec-independent protein export. J. Biol. Chem. 275 16717-22 PubMed
  6. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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