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On One Page

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Gene |

Product(s) |

Expression|

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References |

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Standard Name	sgbE
Gene Synonym(s)	ECK3572, b3583, JW3555, yiaS^[1]
Product Desc.	EG12287^[2]^[3]
Product Synonyms(s)	L-ribulose-5-phosphate 4-epimerase^[1], B3583^[2]^[1], YiaS^[2]^[1], YiaS^[2]^[1] , ECK3572, JW3555, sgbE, b3583
Function from GO	<GO_nr />
Knock-Out Phenotype
Regulation/Expression	transcription unit(s): yiaKLMNO-lyxK-sgbHUE^[2], yiaKLMNOPQRS
Regulation/Activity
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Notes

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Gene

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Product(s)

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Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name	YiaS
Synonyms	L-ribulose-5-phosphate 4-epimerase^[1], B3583^[2]^[1], YiaS^[2]^[1], YiaS^[2]^[1] , ECK3572, JW3555, sgbE, b3583
Product description	EG12287^[2]^[3]
EC number (for enzymes)	5.1.3.4^[1]
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005737	cytoplasm				C	Seeded from Riley et al 2006 ^[1].	Missing: evidence, reference
	GO:0008270	zinc ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004661	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008270	zinc ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0862	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008742	L-ribulose-phosphate 4-epimerase activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004661	F	Seeded from EcoCyc (v14.0)	complete
	GO:0008742	L-ribulose-phosphate 4-epimerase activity	GOA:spec GO_REF:0000003	IEA: Inferred from Electronic Annotation	EC:5.1.3.4	F	Seeded from EcoCyc (v14.0)	complete
	GO:0019572	L-arabinose catabolic process	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR004661	P	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001303	F	Seeded from EcoCyc (v14.0)	complete
	GO:0046872	metal ion binding	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	References	Evidence
Protein	cusR	PMID:16606699	Experiment(s):EBI-1146171
Protein	ytfM	PMID:16606699	Experiment(s):EBI-1146171
Protein	aspS	PMID:16606699	Experiment(s):EBI-1146171
Protein	dnaK	PMID:16606699	Experiment(s):EBI-1146171
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Reference/Source	Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MLEQLKADVL AANLALPAHH LVTFTWGNVS AVDETRQWMV IKPSGVEYDV MTADDMVVVE IASGKVVEGS KKPSSDTPTH LALYRRYAEI GGIVHTHSRH ATIWSQAGLD LPAWGTTHAD YFYGAIPCTR QMTAEEINGE YEYQTGEVII ETFEERGRSP AQIPAVLVHS HGPFAWGKNA ADAVHNAVVL EECAYMGLFS RQLAPQLPAM QNELLDKHYL RKHGANAYYG Q
Length	231
Mol. Wt	25.559 kDa
pI	5.9 (calculated)
Extinction coefficient	43,890 - 44,140 (calc based on 11 Y, 5 W, and 2 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	7..198	PF00596 Class II Aldolase and Adducin N-terminal domain		PMID:19920124
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=sgbE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16131454	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948099	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011702	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P37680	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12287	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12287	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948099	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002168	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2195	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Expression

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Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Cellular Levels

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See Help:Cellular levels

Molecule	Organism or Strain	Value	Units	Experimental Conditions	Assay used	Notes	Reference(s)
Protein	E. coli K-12 MG1655	24	molecules/cell/generation	Medium: MOPS Complete Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	14	molecules/cell/generation	Medium: MOPS Minimal Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling		PMID: 24766808
Protein	E. coli K-12 MG1655	8^a	molecules/cell/generation	Medium: MOPS Complete without Methionine Temperature (°C): 37 Doubling time (min): *Growth phase: OD: other:	Ribosome Profiling	Low confidence in the sequencing data set.	PMID: 24766808
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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)
yiaKLMNO-lyxK-sgbHUE
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Figure courtesy of RegulonDB

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Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Translation and Regulation of Translation

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<protect><gbrowseImage> name=NC_000913:3748089..3748129 source=MG1655 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.

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Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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<protect> Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name	Mutation	Phenotype	Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type	Reference	Notes
microarray	GEO Profiles:b3583 (EcoliWiki Page)	NCBI GEO profiles for sgbE
microarray	GenExpDB:b3583 (EcoliWiki Page)	Summary of data for sgbE from multiple microarray studies
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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name	Resource Type	Description	Source	Notes
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Notes

Accessions Related to yiaS Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc	EcoCyc:EG12287	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2195	Escherichia coli str. K-12 substr. MG1655
EcoliGenExpDB (EcoliWiki Page)	EcoliGenExpDB:b3583
EcoGene	EcoGene:EG12287	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002168	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011702	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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Evolution

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Homologs in Other Organisms

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See Help:Evolution_homologs for help entering or editing information in this section of EcoliWiki.

Organism	Homologs (Statistics)	Comments
Apis mellifera	ENSAPMP00000012633 (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Saccharomyces cerevisiae	YJR024C (score: 1.000; bootstrap: 100%)	From Inparanoid:20070104
Tetraodon nigroviridis	GSTENP00024942001 (score: 1.000; bootstrap: 100%) GSTENP00031118001 (score: 1.000; bootstrap: 100%) GSTENP00020027001 (score: 0.494) GSTENP00014406001 (score: 0.320)	From Inparanoid:20070104
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Do-It-Yourself Web Tools

Notes

Families

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
Pfam (EcoliWiki Page)	PF00596 Class II Aldolase and Adducin N-terminal domain
Superfamily (EcoliWiki Page)	SUPERFAMILY:53639
EcoCyc	EcoCyc:EG12287	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12287	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120002168	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB2195	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0011702	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.00 ^1.01 ^1.02 ^1.03 ^1.04 ^1.05 ^1.06 ^1.07 ^1.08 ^1.09 ^1.10 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 ^2.5 ^2.6 ^2.7 ^2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7

yiaS:On One Page

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Notes

Gene

Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

Expression

Overview

Cellular Levels

Notes

Transcription and Transcriptional Regulation

Notes

Translation and Regulation of Translation

Notes

Turnover and Regulation of Turnover

Notes

Experimental

Notes

Accessions Related to yiaS Expression

Notes

Links

Evolution

Homologs in Other Organisms

Do-It-Yourself Web Tools

Notes

Families

Notes

Links

References

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