rspA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RspA

Synonyms

predicted dehydratase[1], B1581[2][1], RspA[2][1], AltD[3][1], ManD[3][1] , ECK1576, JW1573, b1581

Product description

starvation sensing protein RspA[2]; mannonate/altronate dehydratase[3]

Bifunctional D-altronate/D-mannonate dehydratase; overproduction prevents homoserine lactone-induced synthesis of RpoS[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001354

F

Seeded from EcoCyc (v14.0)

complete

GO:0008152

metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001354

P

Seeded from EcoCyc (v14.0)

complete

GO:0009063

cellular amino acid catabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR018110

P

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

groL

PMID:16606699[5]

Experiment(s):EBI-1140213

Protein

hdeB

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

kdgK

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

thrC

PMID:19402753[6]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKIVKAEVFV TCPGRNFVTL KITTEDGITG LGDATLNGRE LSVASYLQDH LCPQLIGRDA
HRIEDIWQFF YKGAYWRRGP VTMSAISAVD MALWDIKAKA ANMPLYQLLG GASREGVMVY
CHTTGHSIDE ALDDYARHQE LGFKAIRVQC GIPGMKTTYG MSKGKGLAYE PATKGQWPEE
QLWSTEKYLD FMPKLFDAVR NKFGFNEHLL HDMHHRLTPI EAARFGKSIE DYRMFWMEDP
TPAENQECFR LIRQHTVTPI AVGEVFNSIW DCKQLIEEQL IDYIRTTLTH AGGITGMRRI
ADFASLYQVR TGSHGPSDLS PVCMAAALHF DLWVPNFGVQ EYMGYSEQML EVFPHNWTFD
NGYMHPGDKP GLGIEFDEKL AAKYPYEPAY LPVARLEDGT LWNW
Length

404

Mol. Wt

45.968 kDa

pI

6.0 (calculated)

Extinction coefficient

87,320 - 88,195 (calc based on 18 Y, 11 W, and 7 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

128..259

PF01188 Mandelate racemase / muconate lactonizing enzyme, C-terminal domain

PMID:19920124[7]

Domain

6..110

PF02746 Mandelate racemase / muconate lactonizing enzyme, N-terminal domain

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rspA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129539

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946126

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0005275

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P38104

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G6839

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12451

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946126

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003428

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2345

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. 6.0 6.1 6.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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