rpoH:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RpoH

Synonyms

RNA polymerase, sigma 32 (sigma H) factor[1], B3461[2][1], Hin[2][1], Fam[2][1], HtpR[2][1], RpoH[2][1], sigmaH factor[2][1], sigmaH[2][1], sigma32[2][1], sigma H factor[3][1], sigma H[3][1], sigma 32[3][1], sigma 32 factor[3][1] , ECK3445, fam, hin, htpR, JW3426, b3461

Product description

sigma32 factor[2]; RNA polymerase, sigma 32 (sigma H) factor[3];

Component of RNA polymerase sigma32[2]; RNA polymerase sigma 32[3]

RNA polymerase subunit, sigma 32, heat shock transcription[4]

EC number (for enzymes)


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Notes

A list of genes regulated by RpoH may be found on the RNA polymerase sigma32 page.

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000943

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007627

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007630

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012759

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013324

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013325

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014284

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000943

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007627

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007630

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012759

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013324

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013325

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014284

F

Seeded from EcoCyc (v14.0)

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006352

transcription initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000943

P

Seeded from EcoCyc (v14.0)

complete

GO:0006352

transcription initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007627

P

Seeded from EcoCyc (v14.0)

complete

GO:0006352

transcription initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007630

P

Seeded from EcoCyc (v14.0)

complete

GO:0006352

transcription initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012759

P

Seeded from EcoCyc (v14.0)

complete

GO:0006352

transcription initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013324

P

Seeded from EcoCyc (v14.0)

complete

GO:0006352

transcription initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013325

P

Seeded from EcoCyc (v14.0)

complete

GO:0006352

transcription initiation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014284

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000943

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007627

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007630

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012759

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013324

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013325

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014284

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0731

P

Seeded from EcoCyc (v14.0)

complete

GO:0008270

zinc ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012759

F

Seeded from EcoCyc (v14.0)

complete

GO:0009408

response to heat

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012759

P

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000943

F

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007627

F

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007630

F

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012759

F

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013324

F

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013325

F

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR014284

F

Seeded from EcoCyc (v14.0)

complete

GO:0016987

sigma factor activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0731

F

Seeded from EcoCyc (v14.0)

complete

GO:0006950

response to stress

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0346

P

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

PMID:15757896[5]

IDA: Inferred from Direct Assay

F

complete

GO:0016987

sigma factor activity

PMID:6380765[6]

IDA: Inferred from Direct Assay

F

complete

GO:0006355

regulation of transcription, DNA-dependent

PMID:15757896[5]

IMP: Inferred from Mutant Phenotype

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of RNA polymerase sigma32

could be indirect

Protein

yagS

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

rpoA

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

dnaK

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

rpoC

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

clpB

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

rpoB

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

yciM

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

yahB

PMID:16606699[7]

Experiment(s):EBI-1145951

Protein

dnaK

PMID:15690043[8]

Experiment(s):EBI-879426, EBI-883617

Protein

qor

PMID:15690043[8]

Experiment(s):EBI-879426, EBI-883617

Protein

rplC

PMID:15690043[8]

Experiment(s):EBI-879426

Protein

rpoA

PMID:15690043[8]

Experiment(s):EBI-879426

Protein

rpoC

PMID:15690043[8]

Experiment(s):EBI-879426

Protein

rpsD

PMID:15690043[8]

Experiment(s):EBI-879426

Protein

rplL

PMID:15690043[8]

Experiment(s):EBI-883617

Protein

rpoA

PMID:19402753[9]

MALDI(Z-score):35.145614

Protein

qorA

PMID:19402753[9]

LCMS(ID Probability):99.6 MALDI(Z-score):39.239378

Protein

rplL

PMID:19402753[9]

LCMS(ID Probability):99.6

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

<protect></protect>

Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTDKMQSLAL APVGNLDSYI RAANAWPMLS ADEERALAEK LHYHGDLEAA KTLILSHLRF
VVHIARNYAG YGLPQADLIQ EGNIGLMKAV RRFNPEVGVR LVSFAVHWIK AEIHEYVLRN
WRIVKVATTK AQRKLFFNLR KTKQRLGWFN QDEVEMVARE LGVTSKDVRE MESRMAAQDM
TFDLSSDDDS DSQPMAPVLY LQDKSSNFAD GIEDDNWEEQ AANRLTDAMQ GLDERSQDII
RARWLDEDNK STLQELADRY GVSAERVRQL EKNAMKKLRA AIEA
Length

284

Mol. Wt

32.469 kDa

pI

5.7 (calculated)

Extinction coefficient

43,430 (calc based on 7 Y, 6 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

53..123

PF04542 Sigma-70 region 2

PMID:19920124[10]

Domain

228..280

PF04545 Sigma-70, region 4

PMID:19920124[10]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rpoH taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

<protect></protect>

Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131333

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947970

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011303

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AGB3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10897

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10897

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947970

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000888

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0890

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.00 1.01 1.02 1.03 1.04 1.05 1.06 1.07 1.08 1.09 1.10 1.11 1.12 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 2.9 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 3.3 3.4 3.5 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 Zhao, K et al. (2005) The global transcriptional response of Escherichia coli to induced sigma 32 protein involves sigma 32 regulon activation followed by inactivation and degradation of sigma 32 in vivo. J. Biol. Chem. 280 17758-68 PubMed
  6. Grossman, AD et al. (1984) The htpR gene product of E. coli is a sigma factor for heat-shock promoters. Cell 38 383-90 PubMed
  7. 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. 8.0 8.1 8.2 8.3 8.4 8.5 8.6 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. 9.0 9.1 9.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. 10.0 10.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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