rob:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Rob

Synonyms

DNA-binding transcriptional activator [1], B4396 [2][1], RobA [2][1], CbpB [2][1], Rob [2][1], right origin-binding protein [2][1] , cbpB, ECK4388, JW4359, robA, b4396

Product description

Rob transcriptional activator[2][3]

Right oriC-binding protein, AraC family[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012287

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

F

Seeded from EcoCyc (v14.0)

complete

GO:0003700

transcription factor activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020449

F

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

C

Seeded from EcoCyc (v14.0)

complete

GO:0005622

intracellular

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020449

C

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

P

Seeded from EcoCyc (v14.0)

complete

GO:0006355

regulation of transcription, DNA-dependent

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020449

P

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000005

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR020449

F

Seeded from EcoCyc (v14.0)

complete

GO:0043565

sequence-specific DNA binding

PMID:10551881[5]

IDA: Inferred from Direct Assay

F

complete

GO:0045449

regulation of transcription

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012287

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsT

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

ihfA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

phnI

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

polA

PMID:19402753[6]

LCMS(ID Probability):99.6 MALDI(Z-score):5.353895

Protein

hns

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

rpoA

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

hupA

PMID:19402753[6]

LCMS(ID Probability):99.6 MALDI(Z-score):2.522944

Protein

phnG

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

hupB

PMID:19402753[6]

LCMS(ID Probability):99.6

Protein

stpA

PMID:19402753[6]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MDQAGIIRDL LIWLEGHLDQ PLSLDNVAAK AGYSKWHLQR MFKDVTGHAI GAYIRARRLS
KSAVALRLTA RPILDIALQY RFDSQQTFTR AFKKQFAQTP ALYRRSPEWS AFGIRPPLRL
GEFTMPEHKF VTLEDTPLIG VTQSYSCSLE QISDFRHEMR YQFWHDFLGN APTIPPVLYG
LNETRPSQDK DDEQEVFYTT ALAQDQADGY VLTGHPVMLQ GGEYVMFTYE GLGTGVQEFI
LTVYGTCMPM LNLTRRKGQD IERYYPAEDA KAGDRPINLR CELLIPIRR
Length

289

Mol. Wt

33.145 kDa

pI

7.2 (calculated)

Extinction coefficient

42,860 - 43,235 (calc based on 14 Y, 4 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

66..105

PF00165 Bacterial regulatory helix-turn-helix proteins, AraC family

PMID:19920124[7]

Domain

126..288

PF06445 Bacterial transcription activator, effector binding domain

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rob taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16132213

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948916

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0014420

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACI0

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11366

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11366

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948916

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001334

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1340

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Azam, TA & Ishihama, A (1999) Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J. Biol. Chem. 274 33105-13 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 6.9 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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