rffM:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

RffM

Synonyms

UDP-N-acetyl-D-mannosaminuronic acid transferase[1], B3794[2][1], Rff[2][1], RffM[2][1], WecG[2][1] , ECK3787, JW3770, rff, wecG, b3794

Product description

UDP-ManNAcA transferase; needed for ECA (enterobacterial common antigen) synthesis[3]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0009058

biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004629

P

Seeded from EcoCyc (v14.0)

complete

GO:0009246

enterobacterial common antigen biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01001

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

GO:0016757

transferase activity, transferring glycosyl groups

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0328

F

Seeded from EcoCyc (v14.0)

complete

GO:0016758

transferase activity, transferring hexosyl groups

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01001

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

ynbC

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

rpsI

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

recT

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

glpD

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

putA

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

yciH

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

proS

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

dbpA

PMID:16606699[4]

Experiment(s):EBI-1146690

Protein

dnaK

PMID:16606699[4]

Experiment(s):EBI-1146690

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML TIEDNAEVRE
LINAAEFKYA DGISVVRSVR KKYPQAQVSR VAGADLWEEL MARAGKEGTP VFLVGGKPEV
LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ ALFERIHASG AQIVTVAMGS PKQEIIMRDC
RLVHPDALYM GVGGTYDVFT GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW
HYTGNL
Length

246

Mol. Wt

27.928 kDa

pI

9.9 (calculated)

Extinction coefficient

47,900 - 48,025 (calc based on 10 Y, 6 W, and 1 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

60..231

PF03808 Glycosyl transferase WecB/TagA/CpsF family

PMID:19920124[5]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=rffM taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131650

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948301

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0012394

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P27836

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11458

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11458

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948301

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001421

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1427

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  4. 4.0 4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  5. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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