pyrE:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PyrE

Synonyms

orotate phosphoribosyltransferase[1], B3642[2][1], PyrE[2][1] , ECK3632, JW3617, b3642

Product description

PyrE[2][3];

Component of orotate phosphoribosyltransferase[2][3]

Orotate phosphoribosyltransferase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000287

magnesium ion binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01208

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0004588

orotate phosphoribosyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01208

F

Seeded from EcoCyc (v14.0)

complete

GO:0004588

orotate phosphoribosyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004467

F

Seeded from EcoCyc (v14.0)

complete

GO:0004588

orotate phosphoribosyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.4.2.10

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:9222315[5]

IDA: Inferred from Direct Assay

C

complete

GO:0006221

pyrimidine nucleotide biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01208

P

Seeded from EcoCyc (v14.0)

complete

GO:0006221

pyrimidine nucleotide biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004467

P

Seeded from EcoCyc (v14.0)

complete

GO:0004588

orotate phosphoribosyltransferase activity

PMID:9222315[5]

IDA: Inferred from Direct Assay

F

complete

GO:0000287

magnesium ion binding

PMID:7050303[6]

IDA: Inferred from Direct Assay

F

complete

GO:0006221

pyrimidine nucleotide biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0665

P

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

PMID:8620002[7]

IDA: Inferred from Direct Assay

F

complete

GO:0009116

nucleoside metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000836

P

Seeded from EcoCyc (v14.0)

complete

GO:0006221

pyrimidine nucleotide biosynthetic process

PMID:8501045[8]

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of orotate phosphoribosyltransferase

could be indirect

Protein

iscS

PMID:16606699[9]

Experiment(s):EBI-1146389
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKPYQRQFIE FALSKQVLKF GEFTLKSGRK SPYFFNAGLF NTGRDLALLG RFYAEALVDS
GIEFDLLFGP AYKGIPIATT TAVALAEHHD LDLPYCFNRK EAKDHGEGGN LVGSALQGRV
MLVDDVITAG TAIRESMEII QANGATLAGV LISLDRQERG RGEISAIQEV ERDYNCKVIS
IITLKDLIAY LEEKPEMAEH LAAVKAYREE FGV
Length

213

Mol. Wt

23.567 kDa

pI

5.3 (calculated)

Extinction coefficient

11,920 - 12,170 (calc based on 8 Y, 0 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P0A7E3

Domain

37..154

PF00156 Phosphoribosyl transferase domain

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pyrE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131513

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:948157

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011904

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A7E3

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10808

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10808

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:948157

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000799

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0801

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Dodin, G (1981) A rapid purification by affinity chromatography of orotate phosphoribosyltransferase from Escherichia coli K-12. FEBS Lett. 134 20-4 PubMed
  6. ↑ Dodin, G et al. (1982) Role of magnesium cations in the yeast orotate phosphoribosyltransferase catalyzed reaction. Mechanism of the inhibition by Cu++ and Ni++ ions. J. Inorg. Biochem. 16 201-13 PubMed
  7. ↑ Henriksen, A et al. (1996) A flexible loop at the dimer interface is a part of the active site of the adjacent monomer of Escherichia coli orotate phosphoribosyltransferase. Biochemistry 35 3803-9 PubMed
  8. ↑ Jensen, KF (1993) The Escherichia coli K-12 "wild types" W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels. J. Bacteriol. 175 3401-7 PubMed
  9. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  10. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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