potH:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PotH

Synonyms

putrescine transporter subunit: membrane component of ABC superfamily[1], B0856[2][1], PotH[2][1] , ECK0847, JW0840, b0856

Product description

PotH[2][3];

Component of putrescine ABC transporter[2][3]

Putrescine ABC transporter permease[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0015594

putrescine-importing ATPase activity

PMID:8416922[5]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0015594

putrescine-importing ATPase activity

PMID:8416922[5]

F

Seeded from EcoCyc (v14.0)

Missing: evidence

GO:0015847

putrescine transport

PMID:8416922[5]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000515

C

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0043190

ATP-binding cassette (ABC) transporter complex

PMID:8416922[5]

C

Seeded from EcoCyc (v14.0)

Missing: evidence

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of putrescine ABC transporter

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 6 predicted transmembrane domains

Daley et al. (2005) [6]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MSTLEPAAQS KPPGGFKLWL SQLQMKHGRK LVIALPYIWL ILLFLLPFLI VFKISLAEMA
RAIPPYTELM EWADGQLSIT LNLGNFLQLT DDPLYFDAYL QSLQVAAIST FCCLLIGYPL
AWAVAHSKPS TRNILLLLVI LPSWTSFLIR VYAWMGILKN NGVLNNFLLW LGVIDQPLTI
LHTNLAVYIG IVYAYVPFMV LPIYTALIRI DYSLVEAALD LGARPLKTFF TVIVPLTKGG
IIAGSMLVFI PAVGEFVIPE LLGGPDSIMI GRVLWQEFFN NRDWPVASAV AIIMLLLLIV
PIMWFHKHQQ KSVGEHG
Length

317

Mol. Wt

35.491 kDa

pI

8.2 (calculated)

Extinction coefficient

71,390 - 71,640 (calc based on 11 Y, 10 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

113..315

PF00528 Binding-protein-dependent transport system inner membrane component

PMID:19920124[7]

<motif_map/>

tmhmm.php?gene=potH&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=potH taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128824

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945475

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002917

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P31135

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11631

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11631

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945475

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001579

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1588

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 5.2 5.3 Pistocchi, R et al. (1993) Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome. J. Biol. Chem. 268 146-52 PubMed
  6. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  7. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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