potF:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PotF

Synonyms

putrescine transporter subunit: periplasmic-binding component of ABC superfamily[1], B0854[2][1], PotF[2][1] , ECK0845, JW0838, b0854

Product description

PotF[2][3];

Component of putrescine ABC transporter[2][3]

Putrescine ABC transporter periplasmic binding protein[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0015594

putrescine-importing ATPase activity

PMID:8416922[5]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0015594

putrescine-importing ATPase activity

PMID:8416922[5]

F

Seeded from EcoCyc (v14.0)

Missing: evidence

GO:0015847

putrescine transport

PMID:8416922[5]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc (v14.0)

complete

GO:0030288

outer membrane-bounded periplasmic space

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001188

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0574

C

Seeded from EcoCyc (v14.0)

complete

GO:0042597

periplasmic space

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0200

C

Seeded from EcoCyc (v14.0)

complete

GO:0043190

ATP-binding cassette (ABC) transporter complex

PMID:8416922[5]

C

Seeded from EcoCyc (v14.0)

Missing: evidence

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of putrescine ABC transporter

could be indirect

Protein

fur

PMID:16606699[6]

Experiment(s):EBI-1138191

Protein

uspG

PMID:16606699[6]

Experiment(s):EBI-1138191

Protein

fusA

PMID:19402753[7]

MALDI(Z-score):28.221673

Protein

pflB

PMID:19402753[7]

MALDI(Z-score):27.986297

Protein

ahpC

PMID:19402753[7]

MALDI(Z-score):20.931933

Protein

tig

PMID:19402753[7]

MALDI(Z-score):23.014425

Protein

eno

PMID:19402753[7]

MALDI(Z-score):28.712136

Protein

pgk

PMID:19402753[7]

MALDI(Z-score):34.534879

Protein

clpB

PMID:19402753[7]

MALDI(Z-score):27.583822

Protein

rpsD

PMID:19402753[7]

MALDI(Z-score):21.341599

Protein

phoA

PMID:19402753[7]

MALDI(Z-score):17.165331

Protein

rpsC

PMID:19402753[7]

MALDI(Z-score):26.836248

Protein

wrbA

PMID:19402753[7]

MALDI(Z-score):24.054961

Protein

tnaA

PMID:19402753[7]

MALDI(Z-score):26.433080

Protein

groS

PMID:19402753[7]

MALDI(Z-score):22.946739

Protein

rpoA

PMID:19402753[7]

MALDI(Z-score):36.287334

Protein

deoD

PMID:19402753[7]

MALDI(Z-score):20.665300

Protein

udp

PMID:19402753[7]

MALDI(Z-score):29.952477

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

periplasm

From EcoCyc[3]

Periplasm

PMID:9298646[8]

EchoLocation:potF


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MTALNKKWLS GLVAGALMAV SVGTLAAEQK TLHIYNWSDY IAPDTVANFE KETGIKVVYD
VFDSNEVLEG KLMAGSTGFD LVVPSASFLE RQLTAGVFQP LDKSKLPEWK NLDPELLKLV
AKHDPDNKFA MPYMWATTGI GYNVDKVKAV LGENAPVDSW DLILKPENLE KLKSCGVSFL
DAPEEVFATV LNYLGKDPNS TKADDYTGPA TDLLLKLRPN IRYFHSSQYI NDLANGDICV
AIGWAGDVWQ ASNRAKEAKN GVNVSFSIPK EGAMAFFDVF AMPADAKNKD EAYQFLNYLL
RPDVVAHISD HVFYANANKA ATPLVSAEVR ENPGIYPPAD VRAKLFTLKV QDPKIDRVRT
RAWTKVKSGK
Length

370

Mol. Wt

40.84 kDa

pI

6.1 (calculated)

Extinction coefficient

63,370 - 63,620 (calc based on 13 Y, 8 W, and 2 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-26

UniProt Manual:Signal Peptides

UniProt:P31133

Domain

34..306

PF01547 Bacterial extracellular solute-binding protein

PMID:19920124[9]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=potF taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128822

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945480

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002913

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P31133

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11629

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11629

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945480

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001577

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1586

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 Pistocchi, R et al. (1993) Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome. J. Biol. Chem. 268 146-52 PubMed
  6. ↑ 6.0 6.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.00 7.01 7.02 7.03 7.04 7.05 7.06 7.07 7.08 7.09 7.10 7.11 7.12 7.13 7.14 7.15 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  9. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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