potD:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PotD

Synonyms

polyamine transporter subunit[1], periplasmic-binding component of ABC superfamily[1], B1123[2][1], PotD[2][1] , ECK1109, JW1109, b1123

Product description

PotD[2][3];

Component of putrescine/spermidine ABC transporter[2][3]

Spermidine ABC transporter periplasmic binding protein; also binds putrescine[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0015417

polyamine-transporting ATPase activity

PMID:8366082[5]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0015846

polyamine transport

PMID:1939142[6]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0030288

outer membrane-bounded periplasmic space

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001188

C

Seeded from EcoCyc (v14.0)

complete

GO:0030288

outer membrane-bounded periplasmic space

PMID:1939142[6]

IEP: Inferred from Expression Pattern

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of putrescine/spermidine ABC transporter

could be indirect

Protein

ydfK

PMID:16606699[7]

Experiment(s):EBI-1138932

Protein

stpA

PMID:16606699[7]

Experiment(s):EBI-1138932

Protein

ymfG

PMID:16606699[7]

Experiment(s):EBI-1138932

Protein

dtd

PMID:16606699[7]

Experiment(s):EBI-1138932

Protein

tatE

PMID:16606699[7]

Experiment(s):EBI-1138932

Protein

ynaE

PMID:16606699[7]

Experiment(s):EBI-1138932

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

periplasm

From EcoCyc[3]

Periplasm

PMID:1939142[6]

EchoLocation:potD


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MKKWSRHLLA AGALALGMSA AHADDNNTLY FYNWTEYVPP GLLEQFTKET GIKVIYSTYE
SNETMYAKLK TYKDGAYDLV VPSTYYVDKM RKEGMIQKID KSKLTNFSNL DPDMLNKPFD
PNNDYSIPYI WGATAIGVNG DAVDPKSVTS WADLWKPEYK GSLLLTDDAR EVFQMALRKL
GYSGNTTDPK EIEAAYNELK KLMPNVAAFN SDNPANPYME GEVNLGMIWN GSAFVARQAG
TPIDVVWPKE GGIFWMDSLA IPANAKNKEG ALKLINFLLR PDVAKQVAET IGYPTPNLAA
RKLLSPEVAN DKTLYPDAET IKNGEWQNDV GAASSIYEEY YQKLKAGR
Length

348

Mol. Wt

38.868 kDa

pI

5.1 (calculated)

Extinction coefficient

80,790 (calc based on 21 Y, 9 W, and 0 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

motif

1-23

UniProt Manual:Signal Peptides

UniProt:P0AFK9

Domain

31..285

PF01547 Bacterial extracellular solute-binding protein

PMID:19920124[8]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=potD taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129086

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945682

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003789

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AFK9

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10752

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10752

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945682

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000743

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0745

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Kashiwagi, K et al. (1993) Functions of potA and potD proteins in spermidine-preferential uptake system in Escherichia coli. J. Biol. Chem. 268 19358-63 PubMed
  6. 6.0 6.1 6.2 Furuchi, T et al. (1991) Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome. J. Biol. Chem. 266 20928-33 PubMed
  7. 7.0 7.1 7.2 7.3 7.4 7.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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