potB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PotB

Synonyms

polyamine transporter subunit[1], membrane component of ABC superfamily[1], B1125[2][1], PotB[2][1] , ECK1111, JW1111, b1125

Product description

PotB[2][3];

Component of putrescine/spermidine ABC transporter[2][3]

Spermidine/putrescine ABC transporter permease[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0015417

polyamine-transporting ATPase activity

PMID:8366082[5]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0015846

polyamine transport

PMID:1939142[6]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9909

C

Seeded from EcoCyc (v14.0)

complete

GO:0016021

integral to membrane

PMID:1939142[6]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of putrescine/spermidine ABC transporter

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 6 predicted transmembrane domains

Daley et al. (2005) [7]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MIVTIVGWLV LFVFLPNLMI IGTSFLTRDD ASFVKMVFTL DNYTRLLDPL YFEVLLHSLN
MALIATLACL VLGYPFAWFL AKLPHKVRPL LLFLLIVPFW TNSLIRIYGL KIFLSTKGYL
NEFLLWLGVI DTPIRIMFTP SAVIIGLVYI LLPFMVMPLY SSIEKLDKPL LEAARDLGAS
KLQTFIRIII PLTMPGIIAG CLLVMLPAMG LFYVSDLMGG AKNLLIGNVI KVQFLNIRDW
PFGAATSITL TIVMGLMLLV YWRASRLLNK KVELE
Length

275

Mol. Wt

31.064 kDa

pI

9.8 (calculated)

Extinction coefficient

46,410 - 46,660 (calc based on 9 Y, 6 W, and 2 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

73..274

PF00528 Binding-protein-dependent transport system inner membrane component

PMID:19920124[8]

<motif_map/>

tmhmm.php?gene=potB&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=potB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:162135921

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945692

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003794

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AFK4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10750

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10750

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945692

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000741

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0743

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Kashiwagi, K et al. (1993) Functions of potA and potD proteins in spermidine-preferential uptake system in Escherichia coli. J. Biol. Chem. 268 19358-63 PubMed
  6. 6.0 6.1 Furuchi, T et al. (1991) Characteristics of the gene for a spermidine and putrescine transport system that maps at 15 min on the Escherichia coli chromosome. J. Biol. Chem. 266 20928-33 PubMed
  7. Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  8. Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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