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References Suggestions


Standard Name


Gene Synonym(s)

ECK3152, b3164, JW5851, bfl[1], bfl

Product Desc.


Component of polynucleotide phosphorylase[3]; degradosome[3]

Polynucleotide phosphorylase; exoribonuclease; PNPase component of RNA degradosome; cold shock protein required for growth at low temperatures[4]

Product Synonyms(s)

polynucleotide phosphorylase/polyadenylase[1], B3164[2][1], Bfl[2][1], Pnp[2][1], polynucleotide phosphorylase[2][1] , bfl, ECK3152, JW5851, b3164

Function from GO

<GO_nr />

Knock-Out Phenotype

transcription unit(s): rpsO-pnp[2], pnp[2], metY-yhbC-nusA-infB-rbfA-truB-rpsO-pnp[2]

Quick Links

porteco.png EcoCyc.gif regulondb.jpg

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Implicated in degadation of sRNAs[5]

Cold induction occurs by reversal of post-translational autoregulation. The pnp mRNA leader is cleaved by RNase III, allowing PNPase to repress its own translation. Mutation of Gly454 impairs autogenous regulation without affecting catalytic activity. Mutants have elevated OM vesiculation (McBroom, 2006). Pnp can add long polynucleotide tails to mRNAs terminated by Rho factor (Mohanty, 2006). The overexpression of the Pnp S1 domain suppresses the cold growth defect of quadruple cspABEG mutant strain BX04 (Xia, 2001).[4]


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  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. 4.0 4.1 EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Andrade, JM & Arraiano, CM (2008) PNPase is a key player in the regulation of small RNAs that control the expression of outer membrane proteins. RNA 14 543-51 PubMed