pcnB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

PcnB

Synonyms

poly(A) polymerase I[1], B0143[2][1], PcnB[2][1], PAP I[2][1] , ECK0142, JW5808, b0143

Product description

poly(A) polymerase I[2][3]

Poly(A) polymerase; controls plasmid copy number; rare AUU start codon, growth-rate regulated; monomeric[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004652

polynucleotide adenylyltransferase activity

PMID:10361280[5]

IDA: Inferred from Direct Assay

F

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002646

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010206

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc (v14.0)

complete

GO:0004652

polynucleotide adenylyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010206

F

Seeded from EcoCyc (v14.0)

complete

GO:0009451

RNA modification

PMID:10361280[5]

IDA: Inferred from Direct Assay

P

complete

GO:0004652

polynucleotide adenylyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.7.19

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0006397

mRNA processing

PMID:10361280[5]

IDA: Inferred from Direct Assay

P

complete

GO:0006276

plasmid maintenance

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0615

P

Seeded from EcoCyc (v14.0)

complete

GO:0006276

plasmid maintenance

PMID:2537812[6]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006350

transcription

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0804

P

Seeded from EcoCyc (v14.0)

complete

GO:0006378

mRNA polyadenylation

PMID:17040898[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006378

mRNA polyadenylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR010206

P

Seeded from EcoCyc (v14.0)

complete

GO:0009451

RNA modification

PMID:17040898[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0003723

RNA binding

PMID:10361280[5]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0009451

RNA modification

PMID:9435023[8]

IGI: Inferred from Genetic Interaction

EcoliWiki:cca|EcoliWiki:pnp

P

complete

GO:0005737

cytoplasm

PMID:10361280[5]

IDA: Inferred from Direct Assay

C

complete

GO:0005737

cytoplasm

PMID:1438224[9]

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

gltA

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

dps

PMID:19402753[10]

MALDI(Z-score):28.075216

Protein

rplE

PMID:19402753[10]

LCMS(ID Probability):99.6

Protein

rplM

PMID:19402753[10]

MALDI(Z-score):24.173218

Protein

rlmL

PMID:19402753[10]

MALDI(Z-score):18.794422

Protein

rplB

PMID:19402753[10]

MALDI(Z-score):29.552366

Protein

rpsC

PMID:19402753[10]

LCMS(ID Probability):99.6 MALDI(Z-score):32.827078

Protein

rpsD

PMID:19402753[10]

MALDI(Z-score):34.875026

Protein

pssA

PMID:19402753[10]

MALDI(Z-score):24.386749

Protein

rplD

PMID:19402753[10]

MALDI(Z-score):24.466084

Protein

yfiF

PMID:19402753[10]

MALDI(Z-score):30.740581

Protein

rluB

PMID:19402753[10]

MALDI(Z-score):32.442766

Protein

rsmC

PMID:19402753[10]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MLSREESEAE QAVARPQVTV IPREQHAISR KDISENALKV MYRLNKAGYE AWLVGGGVRD
LLLGKKPKDF DVTTNATPEQ VRKLFRNCRL VGRRFRLAHV MFGPEIIEVA TFRGHHEGNV
SDRTTSQRGQ NGMLLRDNIF GSIEEDAQRR DFTINSLYYS VADFTVRDYV GGMKDLKDGV
IRLIGNPETR YREDPVRMLR AVRFAAKLGM RISPETAEPI PRLATLLNDI PPARLFEESL
KLLQAGYGYE TYKLLCEYHL FQPLFPTITR YFTENGDSPM ERIIEQVLKN TDTRIHNDMR
VNPAFLFAAM FWYPLLETAQ KIAQESGLTY HDAFALAMND VLDEACRSLA IPKRLTTLTR
DIWQLQLRMS RRQGKRAWKL LEHPKFRAAY DLLALRAEVE RNAELQRLVK WWGEFQVSAP
PDQKGMLNEL DEEPSPRRRT RRPRKRAPRR EGTA
Length

454

Mol. Wt

52.549 kDa

pI

9.9 (calculated)

Extinction coefficient

53,860 - 54,235 (calc based on 14 Y, 6 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

62..193

PF01743 Poly A polymerase head domain

PMID:19920124[11]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=pcnB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:162135892

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947318

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000493

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ABF1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10690

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10690

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947318

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000683

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0684

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 Raynal, LC & Carpousis, AJ (1999) Poly(A) polymerase I of Escherichia coli: characterization of the catalytic domain, an RNA binding site and regions for the interaction with proteins involved in mRNA degradation. Mol. Microbiol. 32 765-75 PubMed
  6. ↑ Liu, JD & Parkinson, JS (1989) Genetics and sequence analysis of the pcnB locus, an Escherichia coli gene involved in plasmid copy number control. J. Bacteriol. 171 1254-61 PubMed
  7. ↑ 7.0 7.1 Mohanty, BK & Kushner, SR (2006) The majority of Escherichia coli mRNAs undergo post-transcriptional modification in exponentially growing cells. Nucleic Acids Res. 34 5695-704 PubMed
  8. ↑ Mikiewicz, D et al. (1997) Isolation and characterization of a ColE1-like plasmid from Enterobacter agglomerans with a novel variant of rom gene. Plasmid 38 210-9 PubMed
  9. ↑ Cao, GJ & Sarkar, N (1992) Identification of the gene for an Escherichia coli poly(A) polymerase. Proc. Natl. Acad. Sci. U.S.A. 89 10380-4 PubMed
  10. ↑ 10.00 10.01 10.02 10.03 10.04 10.05 10.06 10.07 10.08 10.09 10.10 10.11 10.12 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  11. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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