ndh:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Ndh

Synonyms

respiratory NADH dehydrogenase 2/cupric reductase[1], B1109[2][1], Ndh[2][1], NDH-2[2][1], NADH dhII[2][1] , ECK1095, JW1095, b1109

Product description

NADH dehydrogenase II[2][3]

Respiratory NADH dehydrogenase II; NADH:ubiquinone oxidoreductase II; membrane-bound flavoprotein; cupric reductase[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status

NOT

GO:0005737

cytoplasm

PMID:6784762[5]

IDA: Inferred from Direct Assay

C

Seeded from Riley et al 2006 [1].

NADH dehydrogenase II fractionates with cellular membranes.

complete

GO:0006810

transport

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc (v14.0)

complete

GO:0009061

anaerobic respiration

P

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0050660

FAD binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001327

F

Seeded from EcoCyc (v14.0)

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

rpsC

PMID:16606699[6]

Experiment(s):EBI-1138895

Protein

rpsI

PMID:16606699[6]

Experiment(s):EBI-1138895

Protein

hldD

PMID:16606699[6]

Experiment(s):EBI-1138895

Protein

ompL

PMID:16606699[6]

Experiment(s):EBI-1138895

Protein

rplB

PMID:16606699[6]

Experiment(s):EBI-1138895

Protein

alr

PMID:16606699[6]

Experiment(s):EBI-1138895

Protein

tufB

PMID:19402753[7]

MALDI(Z-score):19.246938

Protein

gnd

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

rfaD

PMID:19402753[7]

MALDI(Z-score):38.374327

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MTTPLKKIVI VGGGAGGLEM ATQLGHKLGR KKKAKITLVD RNHSHLWKPL LHEVATGSLD
EGVDALSYLA HARNHGFQFQ LGSVIDIDRE AKTITIAELR DEKGELLVPE RKIAYDTLVM
ALGSTSNDFN TPGVKENCIF LDNPHQARRF HQEMLNLFLK YSANLGANGK VNIAIVGGGA
TGVELSAELH NAVKQLHSYG YKGLTNEALN VTLVEAGERI LPALPPRISA AAHNELTKLG
VRVLTQTMVT SADEGGLHTK DGEYIEADLM VWAAGIKAPD FLKDIGGLET NRINQLVVEP
TLQTTRDPDI YAIGDCASCP RPEGGFVPPR AQAAHQMATC AMNNILAQMN GKPLKNYQYK
DHGSLVSLSN FSTVGSLMGN LTRGSMMIEG RIARFVYISL YRMHQIALHG YFKTGLMMLV
GSINRVIRPR LKLH
Length

434

Mol. Wt

47.359 kDa

pI

9.2 (calculated)

Extinction coefficient

28,880 - 29,380 (calc based on 12 Y, 2 W, and 4 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P00393

Domain

7..102

PF00070 Pyridine nucleotide-disulphide oxidoreductase

PMID:19920124[8]

Domain

172..266

PF00070 Pyridine nucleotide-disulphide oxidoreductase

PMID:19920124[8]

Domain

7..321

PF07992 Pyridine nucleotide-disulphide oxidoreductase

PMID:19920124[8]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ndh taxon=562,83333 </beststructure>

Models

View models at:

</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16129072

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946792

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003746

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P00393

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10649

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10649

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946792

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000642

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0643

Escherichia coli str. K-12 substr. MG1655

<protect></protect>

Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. Jaworowski, A et al. (1981) Genetic identification and purification of the respiratory NADH dehydrogenase of Escherichia coli. Biochemistry 20 2041-7 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 6.5 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. 7.0 7.1 7.2 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. 8.0 8.1 8.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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