murG:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MurG

Synonyms

N-acetylglucosaminyl transferase[1], B0090[2][1], MurG[2][1] , ECK0091, JW0088, b0090

Product description

N-acetylglucosaminyl transferase; UDP-N-acetylglucosamine:N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase; murein synthesis peripheral membrane protein interacting with cardiolipin[3]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0031234

extrinsic to internal side of plasma membrane

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0050511

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity

PMID:1649817[4]

IDA: Inferred from Direct Assay

F

Membranes overexpressing MurG were isolated and used to assay specific activity of MurG to accumulate lipid II (Table 5).

complete

GO:0005886

plasma membrane

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00033

C

Seeded from EcoCyc (v14.0)

complete

GO:0050511

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity

PMID:1649817[4]

IMP: Inferred from Mutant Phenotype

F

Temperature-sensitive mutant at non-permissive temperature lyses (Figure 2) and accumulates UDP-GlcNAc, UDP-MurNAc-pentapeptide and lipid I (the product of the reaction catalyzed by MraY) (Table 2 & Figure 7).

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997

C

Seeded from EcoCyc (v14.0)

complete

GO:0005886

plasma membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-1003

C

Seeded from EcoCyc (v14.0)

complete

GO:0007049

cell cycle

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0131

P

Seeded from EcoCyc (v14.0)

complete

GO:0008360

regulation of cell shape

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0133

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00033

P

Seeded from EcoCyc (v14.0)

complete

GO:0009252

peptidoglycan biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0573

P

Seeded from EcoCyc (v14.0)

complete

GO:0016020

membrane

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0472

C

Seeded from EcoCyc (v14.0)

complete

GO:0019277

UDP-N-acetylgalactosamine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006009

P

Seeded from EcoCyc (v14.0)

complete

GO:0019898

extrinsic to membrane

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-9903

C

Seeded from EcoCyc (v14.0)

complete

GO:0030246

carbohydrate binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007235

F

Seeded from EcoCyc (v14.0)

complete

GO:0030259

lipid glycosylation

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00033

P

Seeded from EcoCyc (v14.0)

complete

GO:0030259

lipid glycosylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004276

P

Seeded from EcoCyc (v14.0)

complete

GO:0030259

lipid glycosylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR007235

P

Seeded from EcoCyc (v14.0)

complete

GO:0050511

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00033

F

Seeded from EcoCyc (v14.0)

complete

GO:0050511

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006009

F

Seeded from EcoCyc (v14.0)

complete

GO:0050511

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.4.1.227

F

Seeded from EcoCyc (v14.0)

complete

GO:0050511

undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase activity

PMID:1649817[4]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

GO:0051301

cell division

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0132

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

fimD

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

hupA

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

hyfB

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

murE

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

rplK

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

rplL

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

rplM

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

rplS

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

rplX

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

rpmG

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

rpsB

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

yfiF

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

yphH

PMID:15690043[5]

Experiment(s):EBI-881701

Protein

glnD

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

glpD

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

dld

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

yedD

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

rpsD

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

malT

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

rpsI

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

yfaQ

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

cpdB

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

dadA

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

yddW

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

uspG

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

rplQ

PMID:16606699[6]

Experiment(s):EBI-1135548

Protein

tufB

PMID:19402753[7]

MALDI(Z-score):24.210159

Protein

murE

PMID:19402753[7]

LCMS(ID Probability):99.0

Protein

fimD

PMID:19402753[7]

LCMS(ID Probability):99.0

Protein

hyfB

PMID:19402753[7]

LCMS(ID Probability):99.0

Protein

yphH

PMID:19402753[7]

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

plasma membrane

From EcoCyc[8]

Inner Membrane

PMID:8449890[9]

EchoLocation:murG


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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MSGQGKRLMV MAGGTGGHVF PGLAVAHHLM AQGWQVRWLG TADRMEADLV PKHGIEIDFI
RISGLRGKGI KALIAAPLRI FNAWRQARAI MKAYKPDVVL GMGGYVSGPG GLAAWSLGIP
VVLHEQNGIA GLTNKWLAKI ATKVMQAFPG AFPNAEVVGN PVRTDVLALP LPQQRLAGRE
GPVRVLVVGG SQGARILNQT MPQVAAKLGD SVTIWHQSGK GSQQSVEQAY AEAGQPQHKV
TEFIDDMAAA YAWADVVVCR SGALTVSEIA AAGLPALFVP FQHKDRQQYW NALPLEKAGA
AKIIEQPQLS VDAVANTLAG WSRETLLTMA ERARAASIPD ATERVANEVS RVARA
Length

355

Mol. Wt

37.813 kDa

pI

10.1 (calculated)

Extinction coefficient

56,950 - 57,075 (calc based on 5 Y, 9 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P17443

Domain

184..347

PF04101 Glycosyltransferase family 28 C-terminal domain

PMID:19920124[10]

Domain

8..146

PF03033 Glycosyltransferase family 28 N-terminal domain

PMID:19920124[10]

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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=murG taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128083

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946321

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000322

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P17443

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10623

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10623

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946321

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000616

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0618

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  4. ↑ 4.0 4.1 4.2 Mengin-Lecreulx, D et al. (1991) The murG gene of Escherichia coli codes for the UDP-N-acetylglucosamine: N-acetylmuramyl-(pentapeptide) pyrophosphoryl-undecaprenol N-acetylglucosamine transferase involved in the membrane steps of peptidoglycan synthesis. J. Bacteriol. 173 4625-36 PubMed
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 5.11 5.12 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.00 6.01 6.02 6.03 6.04 6.05 6.06 6.07 6.08 6.09 6.10 6.11 6.12 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  9. ↑ Bupp, K & van Heijenoort, J (1993) The final step of peptidoglycan subunit assembly in Escherichia coli occurs in the cytoplasm. J. Bacteriol. 175 1841-3 PubMed
  10. ↑ 10.0 10.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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