mnmA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

TrmU

Synonyms

tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase[1], B1133[2][1], YcfB[2][1], AsuE[2][1], TrmU[2][1], MnmA[2][1] , asuE, ECK1119, JW1119, trmU, ycfB, b1133

Product description

tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase[2][3]

tRNA U34 sulfurtransferase; first step in mnm(5)-s(2)U34-tRNA synthesis; thiolates U34 at C2; YccK(TusE) binding partner; antisuppressor[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000049

tRNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0820

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003723

RNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00144

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004506

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0006400

tRNA modification

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00144

P

Seeded from EcoCyc (v14.0)

complete

GO:0008033

tRNA processing

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004506

P

Seeded from EcoCyc (v14.0)

complete

GO:0008033

tRNA processing

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0819

P

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00144

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004506

F

Seeded from EcoCyc (v14.0)

complete

GO:0016740

transferase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

mltD

PMID:15690043[5]

Experiment(s):EBI-888696

Protein

melR

PMID:15690043[5]

Experiment(s):EBI-888696

Protein

nadE

PMID:16606699[6]

Experiment(s):EBI-1138978

Protein

rplB

PMID:16606699[6]

Experiment(s):EBI-1138978
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIEYAALKFN VVSDPMSETA KKVIVGMSGG VDSSVSAWLL QQQGYQVEGL FMKNWEEDDG
EEYCTAAADL ADAQAVCDKL GIELHTVNFA AEYWDNVFEL FLAEYKAGRT PNPDILCNKE
IKFKAFLEFA AEDLGADYIA TGHYVRRADV DGKSRLLRGL DSNKDQSYFL YTLSHEQIAQ
SLFPVGELEK PQVRKIAEDL GLVTAKKKDS TGICFIGERK FREFLGRYLP AQPGKIITVD
GDEIGEHQGL MYHTLGQRKG LGIGGTKEGT EEPWYVVDKD VENNILVVAQ GHEHPRLMSV
GLIAQQLHWV DREPFTGTMR CTVKTRYRQT DIPCTVKALD DDRIEVIFDE PVAAVTPGQS
AVFYNGEVCL GGGIIEQRLP LPV
Length

383

Mol. Wt

42.638 kDa

pI

4.8 (calculated)

Extinction coefficient

48,360 - 49,235 (calc based on 14 Y, 5 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

6..360

PF03054 tRNA methyl transferase

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mnmA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111218

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945690

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003815

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P25745

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11344

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11344

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945690

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001315

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1320

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ 6.0 6.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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