mcrB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

McrB

Synonyms

5-methylcytosine-specific restriction enzyme McrBC, subunit McrB[1], B4346[2][1], RglB[2][1], McrB[2][1] , ECK4336, JW5871, rglB, b4346

Product description

McrB subunit of 5-methylcytosine restriction system[2][3];

Component of McrBC restriction endonuclease[3]

McrBC restriction endonuclease, catalytic subunit; 5-methylcytosine-specific; requires GTP, inhibited by ATP[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677

DNA binding

PMID:7781618[5]

IDA: Inferred from Direct Assay

F

complete

GO:0006308

DNA catabolic process

PMID:2664457[6]

IDA: Inferred from Direct Assay

P

complete

GO:0000166

nucleotide binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003677

DNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0238

F

Seeded from EcoCyc (v14.0)

complete

GO:0004518

nuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0540

F

Seeded from EcoCyc (v14.0)

complete

GO:0004519

endonuclease activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0255

F

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011704

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0009307

DNA restriction-modification system

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0680

P

Seeded from EcoCyc (v14.0)

complete

GO:0009376

HslUV protease complex

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

C

Seeded from EcoCyc (v14.0)

complete

GO:0016787

hydrolase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0378

F

Seeded from EcoCyc (v14.0)

complete

GO:0016887

ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

GO:0015666

restriction endodeoxyribonuclease activity

PMID:2664457[6]

IDA: Inferred from Direct Assay

F

complete

GO:0016887

ATPase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011704

F

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

GO:0003924

GTPase activity

PMID:1317461[7]

IDA: Inferred from Direct Assay

F

complete

GO:0070011

peptidase activity, acting on L-amino acid peptides

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004491

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of McrBC restriction endonuclease

could be indirect

Protein

yjeF

PMID:15690043[8]

Experiment(s):EBI-891305

Protein

yjeF

PMID:19402753[9]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MRKAYLMESI QPWIEKFIKQ AQQQRSQSTK DYPTSYRNLR VKLSFGYGNF TSIPWFAFLG
EGQEASNGIY PVILYYKDFD ELVLAYGISD TNEPHAQWQF SSDIPKTIAE YFQATSGVYP
KKYGQSYYAC SQKVSQGIDY TRFASMLDNI INDYKLIFNS GKSVIPPMSK TESYCLEDAL
NDLFIPETTI ETILKRLTIK KNIILQGPPG VGKTFVARRL AYLLTGEKAP QRVNMVQFHQ
SYSYEDFIQG YRPNGVGFRR KDGIFYNFCQ QAKEQPEKKY IFIIDEINRA NLSKVFGEVM
MLMEHDKRGE NWSVPLTYSE NDEERFYVPE NVYIIGLMNT ADRSLAVVDY ALRRRFSFID
IEPGFDTPQF RNFLLNKKAE PSFVESLCQK MNELNQEISK EATILGKGFR IGHSYFCCGL
EDGTSPDTQW LNEIVMTDIA PLLEEYFFDD PYKQQKWTNK LLGDS
Length

465

Mol. Wt

53.922 kDa

pI

5.7 (calculated)

Extinction coefficient

76,210 - 76,960 (calc based on 29 Y, 6 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

196..350

PF07728 AAA domain (dynein-related subfamily)

PMID:19920124[10]

Domain

5..155

PF12102 Domain of unknown function (DUF3578)

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mcrB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111737

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949122

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0014250

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P15005

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10574

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10574

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949122

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000567

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0569

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Krüger, T et al. (1995) McrB: a prokaryotic protein specifically recognizing DNA containing modified cytosine residues. EMBO J. 14 2661-9 PubMed
  6. ↑ 6.0 6.1 Ross, TK et al. (1989) Identification of a second polypeptide required for McrB restriction of 5-methylcytosine-containing DNA in Escherichia coli K12. Mol. Gen. Genet. 216 402-7 PubMed
  7. ↑ Sutherland, E et al. (1992) McrBC: a multisubunit GTP-dependent restriction endonuclease. J. Mol. Biol. 225 327-48 PubMed
  8. ↑ Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ 10.0 10.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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