iscU:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

IscU

Synonyms

scaffold protein[1], B2529[2][1], NifU[2][1], YfhN[2][1], IscU[2][1] , ECK2526, JW2513, nifU, yfhN, b2529

Product description

scaffold protein involved in iron-sulfur cluster assembly[2][3]

Iron-sulfur cluster assembly scaffold protein[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005506

iron ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002871

F

Seeded from EcoCyc (v14.0)

complete

GO:0005506

iron ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011339

F

Seeded from EcoCyc (v14.0)

complete

GO:0016226

iron-sulfur cluster assembly

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002871

P

Seeded from EcoCyc (v14.0)

complete

GO:0016226

iron-sulfur cluster assembly

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011339

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

cfa

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

dnaK

PMID:15690043[5]

Experiment(s):EBI-887873

Protein

hscA

PMID:15690043[5]

Experiment(s):EBI-887873, EBI-878924, EBI-893552

Protein

iscS

PMID:15690043[5]

Experiment(s):EBI-887873, EBI-887896, EBI-893552, EBI-893577

Protein

ycfZ

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

damX

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

dedA

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

edd

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

pheP

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

proA

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

sucD

PMID:15690043[5]

Experiment(s):EBI-893552

Protein

iscS

PMID:16606699[6]

Experiment(s):EBI-1143157

Protein

edd

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

proA

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

iscS

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):39.954102

Protein

hscA

PMID:19402753[7]

LCMS(ID Probability):99.6 MALDI(Z-score):30.003742

Protein

sucD

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

cfa

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

damX

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

pheP

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

dedA

PMID:19402753[7]

LCMS(ID Probability):99.6

Protein

ycfZ

PMID:19402753[7]

LCMS(ID Probability):99.6

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MAYSEKVIDH YENPRNVGSF DNNDENVGSG MVGAPACGDV MKLQIKVNDE GIIEDARFKT
YGCGSAIASS SLVTEWVKGK SLDEAQAIKN TDIAEELELP PVKIHCSILA EDAIKAAIAD
YKSKREAK
Length

128

Mol. Wt

13.848 kDa

pI

4.7 (calculated)

Extinction coefficient

11,460 - 11,835 (calc based on 4 Y, 1 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

2..128

PF01592 NifU-like N terminal domain

PMID:19920124[8]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=iscU taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130454

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947002

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008321

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0ACD4

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:G7324

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG13395

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947002

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120003896

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB3176

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.00 5.01 5.02 5.03 5.04 5.05 5.06 5.07 5.08 5.09 5.10 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  6. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 7.4 7.5 7.6 7.7 7.8 7.9 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  8. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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