hypE:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

HypE

Synonyms

carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein[1], HydF[2][1], HypE[2][1], B2730[2][1], HydE[2][1], HydB[2][1] , ECK2725, hydB, JW2700, b2730

Product description

hydrogenase maturation protein[2][3]

Hydrogenases 1,2,3 accessory protein; required for CN ligand synthesis at the metallocenter; converts thiocarbamate at its C-terminal Cys to a thiocyanate[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000728

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016188

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

yafY

PMID:16606699[5]

Experiment(s):EBI-1143767

Protein

dps

PMID:19402753[6]

MALDI(Z-score):25.119400

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MQQLINSLFM EAFANPWLAE QEDQARLDLA QLVAEGDRLA FSTDSYVIDP LFFPGGNIGK
LAICGTANDV AVSGAIPRYL SCGFILEEGL PMETLKAVVT SMAETARAAG IAIVTGDTKV
VQRGAVDKLF INTAGMGAIP ANIHWGAQTL TAGDVLLVSG TLGDHGATIL NLREQLGLDG
ELVSDCAVLT PLIQTLRDIP GVKALRDATR GGVNAVVHEF AAACGCGIEL SEAALPVKPA
VRGVCELLGL DALNFANEGK LVIAVERNAA EQVLAALHSH PLGKDAALIG EVVERKGVRL
AGLYGVKRTL DLPHAEPLPR IC
Length

322

Mol. Wt

33.712 kDa

pI

4.9 (calculated)

Extinction coefficient

15,470 - 16,345 (calc based on 3 Y, 2 W, and 7 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

34..132

PF00586 AIR synthase related protein, N-terminal domain

PMID:19920124[7]

Domain

165..315

PF02769 AIR synthase related protein, C-terminal domain

PMID:19920124[7]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=hypE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:226524740

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947182

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008967

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P24193

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10487

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10487

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947182

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000480

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0482

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  6. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  7. ↑ 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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