ffh:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Ffh

Synonyms

Signal Recognition Particle (SRP) component with 4.5S RNA (ffs)[1], B2610[2][1], Ffh[2][1], P48[2][1] , ECK2607, JW5414, b2610

Product description

protein component of the signal recognition particle (SRP)[2][3];

Component of Signal Recognition Particle Protein Translocation System[2][3]

Signal Recognition Particle (SRP) protein, with 4.5S RNA; GTPase involved in co-translational protein translocation into and through membranes[4]

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0048500

signal recognition particle

PMID:1701272[5]

IMP: Inferred from Mutant Phenotype

C

complete

GO:0005525

GTP binding

PMID:7612669[6]

IDA: Inferred from Direct Assay

F

complete

GO:0006612

protein targeting to membrane

PMID:1331806[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:1990904

ribonucleoprotein complex

PMID:1701272[5]

IDA: Inferred from Direct Assay

C

complete

GO:0048500

signal recognition particle

PMID:1331806[7]

IMP: Inferred from Mutant Phenotype

C

complete

GO:0003723

RNA binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0694

F

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0003924

GTPase activity

PMID:7612669[6]

IDA: Inferred from Direct Assay

F

4.5S RNA

complete

GO:0005515

protein binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013822

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000897

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013822

F

Seeded from EcoCyc (v14.0)

complete

GO:0005525

GTP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0342

F

Seeded from EcoCyc (v14.0)

complete

GO:0006614

SRP-dependent cotranslational protein targeting to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004125

P

Seeded from EcoCyc (v14.0)

complete

GO:0006614

SRP-dependent cotranslational protein targeting to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004780

P

Seeded from EcoCyc (v14.0)

complete

GO:0006614

SRP-dependent cotranslational protein targeting to membrane

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR013822

P

Seeded from EcoCyc (v14.0)

complete

GO:0008312

7S RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004125

F

Seeded from EcoCyc (v14.0)

complete

GO:0008312

7S RNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004780

F

Seeded from EcoCyc (v14.0)

complete

GO:0017111

nucleoside-triphosphatase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR003593

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of Signal Recognition Particle Protein Translocation System

could be indirect

Protein

adhE

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

clpA

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

lon

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

metK

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rplA

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rplD

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rplJ

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rpsB

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rpsE

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rpsK

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

tufA

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rpsD

PMID:15690043[8]

Experiment(s):EBI-880876

Protein

rplJ

PMID:19402753[9]

MALDI(Z-score):18.938026

Protein

metK

PMID:19402753[9]

MALDI(Z-score):25.833537

Protein

rpsD

PMID:19402753[9]

MALDI(Z-score):27.913213

Protein

rplA

PMID:19402753[9]

MALDI(Z-score):27.046126

Protein

lon

PMID:19402753[9]

MALDI(Z-score):22.248267

Protein

tufB

PMID:19402753[9]

MALDI(Z-score):22.629013

Protein

clpA

PMID:19402753[9]

MALDI(Z-score):24.365879

RNA

4.5S RNA encoded by ffs gene

Poritz MA, Bernstein HD, Strub K, Zopf D, Wilhelm H, and Walter P. (1990) [5]


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MFDNLTDRLS RTLRNISGRG RLTEDNVKDT LREVRMALLE ADVALPVVRE FINRVKEKAV
GHEVNKSLTP GQEFVKIVRN ELVAAMGEEN QTLNLAAQPP AVVLMAGLQG AGKTTSVGKL
GKFLREKHKK KVLVVSADVY RPAAIKQLET LAEQVGVDFF PSDVGQKPVD IVNAALKEAK
LKFYDVLLVD TAGRLHVDEA MMDEIKQVHA SINPVETLFV VDAMTGQDAA NTAKAFNEAL
PLTGVVLTKV DGDARGGAAL SIRHITGKPI KFLGVGEKTE ALEPFHPDRI ASRILGMGDV
LSLIEDIESK VDRAQAEKLA SKLKKGDGFD LNDFLEQLRQ MKNMGGMASL MGKLPGMGQI
PDNVKSQMDD KVLVRMEAII NSMTMKERAK PEIIKGSRKR RIAAGCGMQV QDVNRLLKQF
DDMQRMMKKM KKGGMAKMMR SMKGMMPPGF PGR
Length

453

Mol. Wt

49.787 kDa

pI

9.9 (calculated)

Extinction coefficient

2,980 - 3,105 (calc based on 2 Y, 0 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

100..296

PF00448 SRP54-type protein, GTPase domain

PMID:19920124[10]

Domain

5..82

PF02881 SRP54-type protein, helical bundle domain

PMID:19920124[10]

Domain

328..427

PF02978 Signal peptide binding domain

PMID:19920124[10]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ffh taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16130531

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947102

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008586

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AGD7

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10300

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10300

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947102

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000294

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0296

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Poritz, MA et al. (1990) An E. coli ribonucleoprotein containing 4.5S RNA resembles mammalian signal recognition particle. Science 250 1111-7 PubMed
  6. ↑ 6.0 6.1 Samuelsson, T et al. (1995) The GTPase activity of the Escherichia coli Ffh protein is important for normal growth. Biochim. Biophys. Acta 1267 83-91 PubMed
  7. ↑ 7.0 7.1 Phillips, GJ & Silhavy, TJ (1992) The E. coli ffh gene is necessary for viability and efficient protein export. Nature 359 744-6 PubMed
  8. ↑ 8.00 8.01 8.02 8.03 8.04 8.05 8.06 8.07 8.08 8.09 8.10 8.11 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  9. ↑ 9.0 9.1 9.2 9.3 9.4 9.5 9.6 Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  10. ↑ 10.0 10.1 10.2 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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