dam:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

Dam

Synonyms

DNA adenine methylase[1], B3387[2][1], Dam[2][1] , ECK3374, JW3350, b3387

Product description

DNA adenine methylase[2][3]

DNA-(adenine-N6)-methyltransferase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

PMID:7037767[5]

IDA: Inferred from Direct Assay

F

complete

GO:0006298

mismatch repair

PMID:6225697[6]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0003676

nucleic acid binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002052

F

Seeded from EcoCyc (v14.0)

complete

GO:0006298

mismatch repair

PMID:6308634[7]

IDA: Inferred from Direct Assay

P

complete

GO:0003677

DNA binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002294

F

Seeded from EcoCyc (v14.0)

complete

GO:0006261

DNA-dependent DNA replication

PMID:3891329[8] PMID:3891330[9]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006306

DNA methylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002294

P

Seeded from EcoCyc (v14.0)

complete

GO:0006306

DNA methylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012326

P

Seeded from EcoCyc (v14.0)

complete

GO:0006306

DNA methylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012327

P

Seeded from EcoCyc (v14.0)

complete

GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002294

F

Seeded from EcoCyc (v14.0)

complete

GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012326

F

Seeded from EcoCyc (v14.0)

complete

GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR012327

F

Seeded from EcoCyc (v14.0)

complete

GO:0009007

site-specific DNA-methyltransferase (adenine-specific) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.1.1.72

F

Seeded from EcoCyc (v14.0)

complete

GO:0032259

methylation

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002052

P

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

aceE

PMID:15690043[10]

Experiment(s):EBI-881035

Protein

lpdA

PMID:15690043[10]

Experiment(s):EBI-881035, EBI-887858

Protein

yjjI

PMID:15690043[10]

Experiment(s):EBI-881035

Protein

glpD

PMID:15690043[10]

Experiment(s):EBI-887858

Protein

rplV

PMID:15690043[10]

Experiment(s):EBI-887858

Protein

serS

PMID:15690043[10]

Experiment(s):EBI-887858

Protein

rplS

PMID:15690043[10]

Experiment(s):EBI-887858

Protein

envY

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

ulaF

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rpsC

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

htpG

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rpsG

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rpoC

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rplQ

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

ybaB

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rpsM

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

yjbD

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

narZ

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rplD

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

groL

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rplA

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rpoB

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

rpsD

PMID:16606699[11]

Experiment(s):EBI-1145744

Protein

glpD

PMID:19402753[12]

LCMS(ID Probability):99.0

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MKKNRAFLKW AGGKYPLLDD IKRHLPKGEC LVEPFVGAGS VFLNTDFSRY ILADINSDLI
SLYNIVKMRT DEYVQAAREL FVPETNCAEV YYQFREEFNK SQDPFRRAVL FLYLNRYGYN
GLCRYNLRGE FNVPFGRYKK PYFPEAELYH FAEKAQNAFF YCESYADSMA RADDASVVYC
DPPYAPLSAT ANFTAYHTNS FTLEQQAHLA EIAEGLVERH IPVLISNHDT MLTREWYQRA
KLHVVKVRRS ISSNGGTRKK VDELLALYKP GVVSPAKK
Length

278

Mol. Wt

32.1 kDa

pI

8.8 (calculated)

Extinction coefficient

40,800 - 41,425 (calc based on 20 Y, 2 W, and 5 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

10..251

PF02086 D12 class N6 adenine-specific DNA methyltransferase

PMID:19920124[13]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=dam taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16131265

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:947893

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011063

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0AEE8

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10204

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10204

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:947893

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000198

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0200

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Herman, GE & Modrich, P (1982) Escherichia coli dam methylase. Physical and catalytic properties of the homogeneous enzyme. J. Biol. Chem. 257 2605-12 PubMed
  6. ↑ Pukkila, PJ et al. (1983) Effects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli. Genetics 104 571-82 PubMed
  7. ↑ Lu, AL et al. (1983) Methyl-directed repair of DNA base-pair mismatches in vitro. Proc. Natl. Acad. Sci. U.S.A. 80 4639-43 PubMed
  8. ↑ Smith, DW et al. (1985) Importance of state of methylation of oriC GATC sites in initiation of DNA replication in Escherichia coli. EMBO J. 4 1319-26 PubMed
  9. ↑ Messer, W et al. (1985) Effect of dam methylation on the activity of the E. coli replication origin, oriC. EMBO J. 4 1327-32 PubMed
  10. ↑ 10.0 10.1 10.2 10.3 10.4 10.5 10.6 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  11. ↑ 11.00 11.01 11.02 11.03 11.04 11.05 11.06 11.07 11.08 11.09 11.10 11.11 11.12 11.13 11.14 11.15 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  12. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  13. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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