ccmF:Gene Product(s)

Standard name	CcmF
Synonyms	heme lyase, CcmF subunit^[1], B2196^[2]^[1], YejR^[2]^[1], CcmF^[2]^[1] , ECK2188, JW2184, yejR, b2196
Product description	cytochrome c-type biogenesis protein^[2]^[3]; Component of [[:Category:Complex:CcmABCDEFGH cytochrome c biogenesis system\|CcmABCDEFGH cytochrome c biogenesis system]]^[3] Heme lyase, with CcmH, required for transfer of heme to apocytochrome from heme-CcmE^[4]
EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference	Evidence Code	with/from	Aspect	Notes	Status
	GO:0005515	protein binding	PMID:11744735^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P0ABM9	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005515	protein binding	PMID:11744735^[5]	IPI: Inferred from Physical Interaction	UniProtKB:P69490	F	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0997	C	Seeded from EcoCyc (v14.0)	complete
	GO:0005886	plasma membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-1003	C	Seeded from EcoCyc (v14.0)	complete
	GO:0008535	respiratory chain complex IV assembly	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002541	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015232	heme transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003567	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015232	heme transporter activity	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003568	F	Seeded from EcoCyc (v14.0)	complete
	GO:0015886	heme transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003567	P	Seeded from EcoCyc (v14.0)	complete
	GO:0015886	heme transport	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003568	P	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR002541	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003567	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003568	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016020	membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0472	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc (v14.0)	complete
	GO:0016021	integral to membrane	GO_REF:0000023	IEA: Inferred from Electronic Annotation	SP_SL:SL-9909	C	Seeded from EcoCyc (v14.0)	complete
	GO:0017004	cytochrome complex assembly	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003567	P	Seeded from EcoCyc (v14.0)	complete
	GO:0017004	cytochrome complex assembly	GOA:interpro GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR003568	P	Seeded from EcoCyc (v14.0)	complete
	GO:0017004	cytochrome complex assembly	GOA:spkw SP_KW:GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0201	P	Seeded from EcoCyc (v14.0)	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
Protein	Subunits of [[:Category:Complex:CcmABCDEFGH cytochrome c biogenesis system\|CcmABCDEFGH cytochrome c biogenesis system]]	could be indirect
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</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence
plasma membrane	C-terminus localized in the cytoplasm with 15 predicted transmembrane domains	Daley et al. (2005) ^[6]
plasma membrane
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence	at EcoCyc MMPEIGNGLL CLALGIALLL SVYPLWGVAR GDARMMASSR LFAWLLFMSV AGAFLVLVNA FVVNDFTVTY VASNSNTQLP VWYRVAATWG AHEGSLLLWV LLMSGWTFAV AIFSQRIPLD IVARVLAIMG MVSVGFLLFI LFTSNPFSRT LPNFPIEGRD LNPLLQDPGL IFHPPLLYMG YVGFSVAFAF AIASLLSGRL DSTYARFTRP WTLAAWIFLT LGIVLGSAWA YYELGWGGWW FWDPVENASF MPWLVGTALM HSLAVTEQRA SFKAWTLLLA ISAFSLCLLG TFLVRSGVLV SVHAFASDPA RGMFILAFMV LVIGGSLLLF AARGHKVRSR VNNALWSRES LLLANNVLLV AAMLVVLLGT LLPLVHKQLG LGSISIGEPF FNTMFTWLMV PFALLLGVGP LVRWGRDRPR KIRNLLIIAF ISTLVLSLLL PWLFESKVVA MTVLGLAMAC WIAVLAIAEA ALRISRGTKT TFSYWGMVAA HLGLAVTIVG IAFSQNYSVE RDVRMKSGDS VDIHEYRFTF RDVKEVTGPN WRGGVATIGV TRDGKPETVL YAEKRYYNTA GSMMTEAAID GGITRDLYAA LGEELENGAW AVRLYYKPFV RWIWAGGLMM ALGGLLCLFD PRYRKRVSPQ KTAPEAV
Length	647
Mol. Wt	71.391 kDa
pI	10.0 (calculated)
Extinction coefficient	164,320 - 164,820 (calc based on 18 Y, 25 W, and 4 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes	References
Domain	89..299	PF01578 Cytochrome C assembly protein		PMID:19920124^[7]
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<motif_map/>

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Structure
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Structures

<beststructure> gene=ccmF taxon=562,83333 </beststructure>

Models

View models at:

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</protect>

Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
NCBI (Protein) (EcoliWiki Page)	GI:16130133	Escherichia coli str. K-12 substr. MG1655
NCBI (Protein) (EcoliWiki Page)	GeneID:948783	Escherichia coli str. K-12 substr. MG1655
ASAP	ASAP:ABE-0007269	Escherichia coli str. K-12 substr. MG1655
UniProt (EcoliWiki Page)	UniProtKB/Swiss-Prot:P33927	Escherichia coli str. K-12 substr. MG1655
EcoCyc	EcoCyc:EG12054	Escherichia coli str. K-12 substr. MG1655
EcoGene	EcoGene:EG12054	Escherichia coli str. K-12 substr. MG1655
NCBI (Gene) (EcoliWiki Page)	GeneID:948783	Escherichia coli str. K-12 substr. MG1655
RegulonDB	RegulonDB:ECK120001967	Escherichia coli str. K-12 substr. MG1655
EchoBASE	EchoBASE:EB1985	Escherichia coli str. K-12 substr. MG1655
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 Ren, Q et al. (2002) A bacterial cytochrome c heme lyase. CcmF forms a complex with the heme chaperone CcmE and CcmH but not with apocytochrome c. J. Biol. Chem. 277 7657-63 PubMed
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

ccmF:Gene Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

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