carA:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

CarA

Synonyms

carbamoyl phosphate synthetase small subunit, glutamine amidotransferase[1], B0032[2][1], Arg[2][1], Cap[2][1], PyrA[2][1], α chain[2][1], small chain[2][1], CarA[2][1] , arg, cap, ECK0033, JW0030, pyrA, b0032

Product description

CarA[2][3];

Component of carbamoyl phosphate synthetase[2][3]

Carbamoyl phosphate synthase, small subunit; glutamine-hydrolyzing, tetramer of heterodimers[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

Missing: evidence, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000991

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001317

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006220

F

Seeded from EcoCyc (v14.0)

complete

GO:0003824

catalytic activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011702

F

Seeded from EcoCyc (v14.0)

complete

GO:0004086

carbamoyl-phosphate synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR002474

F

Seeded from EcoCyc (v14.0)

complete

GO:0004086

carbamoyl-phosphate synthase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006274

F

Seeded from EcoCyc (v14.0)

complete

GO:0004088

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01209

F

Seeded from EcoCyc (v14.0)

complete

GO:0004088

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.3.5.5

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:2682645[5]

IDA: Inferred from Direct Assay

C

complete

GO:0005515

protein binding

PMID:2682645[5]

IMP: Inferred from Mutant Phenotype

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01209

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0005829

cytosol

PMID:16858726[6]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc (v14.0)

complete

GO:0005515

protein binding

PMID:2682645[5]

IMP: Inferred from Mutant Phenotype

F

CarB

complete

GO:0006221

pyrimidine nucleotide biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01209

P

Seeded from EcoCyc (v14.0)

complete

Contributes to

GO:0004088

carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity

PMID:1827118[7]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0006221

pyrimidine nucleotide biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0665

P

Seeded from EcoCyc (v14.0)

complete

GO:0006541

glutamine metabolic process

PMID:1827118[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006526

arginine biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01209

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0055

P

Seeded from EcoCyc (v14.0)

complete

GO:0006526

arginine biosynthetic process

PMID:5319709[8]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006541

glutamine metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001317

P

Seeded from EcoCyc (v14.0)

complete

GO:0006207

'de novo' pyrimidine base biosynthetic process

PMID:5319709[8]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006541

glutamine metabolic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR011702

P

Seeded from EcoCyc (v14.0)

complete

GO:0006541

glutamine metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0315

P

Seeded from EcoCyc (v14.0)

complete

GO:0016874

ligase activity

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0436

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of carbamoyl phosphate synthetase

could be indirect

Protein

carB

PMID:15690043[9]

Experiment(s):EBI-880588, EBI-886422

Protein

murE

PMID:15690043[9]

Experiment(s):EBI-888940

Protein

carB

PMID:19402753[10]

LCMS(ID Probability):99.6 MALDI(Z-score):38.299212

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MIKSALLVLE DGTQFHGRAI GATGSAVGEV VFNTSMTGYQ EILTDPSYSR QIVTLTYPHI
GNVGTNDADE ESSQVHAQGL VIRDLPLIAS NFRNTEDLSS YLKRHNIVAI ADIDTRKLTR
LLREKGAQNG CIIAGDNPDA ALALEKARAF PGLNGMDLAK EVTTAEAYSW TQGSWTLTGG
LPEAKKEDEL PFHVVAYDFG AKRNILRMLV DRGCRLTIVP AQTSAEDVLK MNPDGIFLSN
GPGDPAPCDY AITAIQKFLE TDIPVFGICL GHQLLALASG AKTVKMKFGH HGGNHPVKDV
EKNVVMITAQ NHGFAVDEAT LPANLRVTHK SLFDGTLQGI HRTDKPAFSF QGHPEASPGP
HDAAPLFDHF IELIEQYRKT AK
Length

382

Mol. Wt

41.431 kDa

pI

6.3 (calculated)

Extinction coefficient

22,920 - 23,420 (calc based on 8 Y, 2 W, and 4 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

3..133

PF00988 Carbamoyl-phosphate synthase small chain, CPSase domain

PMID:19920124[11]

Domain

194..373

PF00117 Glutamine amidotransferase class-I

PMID:19920124[11]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=carA taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128026

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000118

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P0A6F1

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10134

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10134

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:949025

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000130

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:949025

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0132

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 1.7 1.8 1.9 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Guillou, F et al. (1989) Escherichia coli carbamoyl-phosphate synthetase: domains of glutaminase and synthetase subunit interaction. Proc. Natl. Acad. Sci. U.S.A. 86 8304-8 PubMed
  6. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  7. ↑ 7.0 7.1 Mullins, LS et al. (1991) Alterations in the energetics of the carbamoyl phosphate synthetase reaction by site-directed modification of the essential sulfhydryl group. J. Biol. Chem. 266 8236-40 PubMed
  8. ↑ 8.0 8.1 Piérard, A & Wiame, JM (1964) Regulation and mutation affecting a glutamine dependent formation of carbamyl phosphate in Escherichia coli. Biochem. Biophys. Res. Commun. 15 76-81 PubMed
  9. ↑ 9.0 9.1 Butland, G et al. (2005) Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433 531-7 PubMed
  10. ↑ Hu, P et al. (2009) Global functional atlas of Escherichia coli encompassing previously uncharacterized proteins. PLoS Biol. 7 e96 PubMed
  11. ↑ 11.0 11.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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