btuF:Gene Product(s)
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| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.
| Standard name |
BtuF |
|---|---|
| Synonyms |
vitamin B12 transporter subunit: periplasmic-binding component of ABC superfamily[1], B0158[2][1], BtuF[2][1], YadT[2][1] , ECK0157, JW0154, yadT, b0158 |
| Product description |
periplasmic vitamin B12 binding protein[2][3]; Component of vitamin B12 ABC transporter[2][3]; vitamin B12 transport system[3] B12 uptake, ABC transporter periplasmic binding protein[4] |
| EC number (for enzymes) |
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| edit table |
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Notes
Function
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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.
| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005381 |
iron ion transmembrane transporter activity |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
F |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006810 |
transport |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0006827 |
high-affinity iron ion transport |
GOA:interpro |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015889 |
cobalamin transport |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
P |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0042597 |
periplasmic space |
IDA: Inferred from Direct Assay |
C |
complete | ||||
| GO:0042597 |
periplasmic space |
GOA:hamap |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0031419 |
cobalamin binding |
IDA: Inferred from Direct Assay |
F |
Binding assay was performed with CN-[57Co]Cbl |
complete | |||
| GO:0042597 |
periplasmic space |
GOA:spkw |
IEA: Inferred from Electronic Annotation |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0042597 |
periplasmic space |
IEA: Inferred from Electronic Annotation |
SP_SL:SL-0200 |
C |
Seeded from EcoCyc (v14.0) |
complete | ||
| GO:0015889 |
cobalamin transport |
IMP: Inferred from Mutant Phenotype |
P |
Table 3 and Figure 5 |
complete | |||
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.
| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
|
Protein |
Subunits of vitamin B12 ABC transporter |
could be indirect |
||
|
Protein |
Subunits of vitamin B12 transport system |
could be indirect |
||
|
Small Molecule |
cobalamin (vitamin B12) |
via filter binding assay AND ITC Kd= 15nM |
| |
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Notes
Localization
See Help:Product_localization for how to add or edit information in this section of EcoliWiki.
| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
|
Periplasm |
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|
Periplasm |
osmotic shock treatment |
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Notes
Structure and Physical Properties
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Physical Properties
See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.
| Name | |
|---|---|
| Sequence |
MAKSLFRALV ALSFLAPLWL NAAPRVITLS PANTELAFAA GITPVGVSSY SDYPPQAQKI EQVSTWQGMN LERIVALKPD LVIAWRGGNA ERQVDQLASL GIKVMWVDAT SIEQIANALR QLAPWSPQPD KAEQAAQSLL DQYAQLKAQY ADKPKKRVFL QFGINPPFTS GKESIQNQVL EVCGGENIFK DSRVPWPQVS REQVLARSPQ AIVITGGPDQ IPKIKQYWGE QLKIPVIPLT SDWFERASPR IILAAQQLCN ALSQVD |
| Length |
266 |
| Mol. Wt |
29.366 kDa |
| pI |
9.1 (calculated) |
| Extinction coefficient |
51,450 - 51,700 (calc based on 5 Y, 8 W, and 2 C residues) |
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Domains/Motifs/Modification Sites
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<motif_map/> |
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Structure
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Structure figures<protect> | ||||||
Notes
Gene Product Resources
See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.
| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
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Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
|
Escherichia coli str. K-12 substr. MG1655 | ||
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Notes
Links
| Name | URL | Comments |
|---|---|---|
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
- ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Cadieux, N et al. (2002) Identification of the periplasmic cobalamin-binding protein BtuF of Escherichia coli. J. Bacteriol. 184 706-17 PubMed
- ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed
Categories
- GO:0005381 ! iron ion transmembrane transporter activity
- GO:0006810 ! transport
- GO:0006827 ! high-affinity iron ion transmembrane transport
- GO:0015889 ! cobalamin transport
- GO:0042597 ! periplasmic space
- GO:0031419 ! cobalamin binding
- Proteins
- Complex:vitamin B12 ABC transporter
- Complex:vitamin B12 transport system
- Products with structures in PDB
- Pfam:PF01497 ! Periplasmic binding protein
