asnB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AsnB

Synonyms

asparagine synthetase B[1], B0674[2][1], AsnB[2][1] , ECK0662, JW0660, b0674

Product description

AsnB[2][3];

Component of asparagine synthetase B[2][3]

Asparagine synthase B [glutamine-hydrolyzing][4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0004066

asparagine synthase (glutamine-hydrolyzing) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001962

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

PMID:6102982[5]

IDA: Inferred from Direct Assay

C

complete

GO:0004066

asparagine synthase (glutamine-hydrolyzing) activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006426

F

Seeded from EcoCyc (v14.0)

complete

GO:0008652

cellular amino acid biosynthetic process

PMID:6102982[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0004066

asparagine synthase (glutamine-hydrolyzing) activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:6.3.5.4

F

Seeded from EcoCyc (v14.0)

complete

GO:0006529

asparagine biosynthetic process

PMID:6102982[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0009063

cellular amino acid catabolic process

PMID:6102982[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006529

asparagine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001962

P

Seeded from EcoCyc (v14.0)

complete

GO:0004066

asparagine synthase (glutamine-hydrolyzing) activity

PMID:6102982[5]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0006529

asparagine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR006426

P

Seeded from EcoCyc (v14.0)

complete

GO:0006529

asparagine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0061

P

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

PMID:8691431[6]

IDA: Inferred from Direct Assay

F

complete

GO:0006541

glutamine metabolic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0315

P

Seeded from EcoCyc (v14.0)

complete

GO:0006541

glutamine metabolic process

PMID:6102982[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0016597

amino acid binding

PMID:8691431[6]

IDA: Inferred from Direct Assay

F

L-glutamine (CHEBI:18050) & L-aspartic acid (CHEBI:17053)

complete

GO:0005737

cytoplasm

PMID:4895361[7]

IDA: Inferred from Direct Assay

C

complete

GO:0016597

amino acid binding

PMID:8691431[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc (v14.0)

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of asparagine synthetase B

could be indirect

Protein

nadE

PMID:16606699[8]

Experiment(s):EBI-1137650

Protein

yjjI

PMID:16606699[8]

Experiment(s):EBI-1137650

Protein

dnaK

PMID:16606699[8]

Experiment(s):EBI-1137650
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MCSIFGVFDI KTDAVELRKK ALELSRLMRH RGPDWSGIYA SDNAILAHER LSIVDVNAGA
QPLYNQQKTH VLAVNGEIYN HQALRAEYGD RYQFQTGSDC EVILALYQEK GPEFLDDLQG
MFAFALYDSE KDAYLIGRDH LGIIPLYMGY DEHGQLYVAS EMKALVPVCR TIKEFPAGSY
LWSQDGEIRS YYHRDWFDYD AVKDNVTDKN ELRQALEDSV KSHLMSDVPY GVLLSGGLDS
SIISAITKKY AARRVEDQER SEAWWPQLHS FAVGLPGSPD LKAAQEVANH LGTVHHEIHF
TVQEGLDAIR DVIYHIETYD VTTIRASTPM YLMSRKIKAM GIKMVLSGEG SDEVFGGYLY
FHKAPNAKEL HEETVRKLLA LHMYDCARAN KAMSAWGVEA RVPFLDKKFL DVAMRINPQD
KMCGNGKMEK HILRECFEAY LPASVAWRQK EQFSDGVGYS WIDTLKEVAA QQVSDQQLET
ARFRFPYNTP TSKEAYLYRE IFEELFPLPS AAECVPGGPS VACSSAKAIE WDEAFKKMDD
PSGRAVGVHQ SAYK
Length

554

Mol. Wt

62.659 kDa

pI

5.7 (calculated)

Extinction coefficient

92,710 - 93,710 (calc based on 29 Y, 9 W, and 8 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P22106

Domain

211..450

PF00733 Asparagine synthase

PMID:19920124[9]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=asnB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128650

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:945281

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0002292

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P22106

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10092

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10092

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:945281

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000088

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0090

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 Humbert, R & Simoni, RD (1980) Genetic and biomedical studies demonstrating a second gene coding for asparagine synthetase in Escherichia coli. J. Bacteriol. 142 212-20 PubMed
  6. ↑ 6.0 6.1 6.2 Parr, IB et al. (1996) Mapping the aspartic acid binding site of Escherichia coli asparagine synthetase B using substrate analogs. J. Med. Chem. 39 2367-78 PubMed
  7. ↑ Cedar, H & Schwartz, JH (1969) The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products. J. Biol. Chem. 244 4112-21 PubMed
  8. ↑ 8.0 8.1 8.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  9. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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