araB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

AraB

Synonyms

L-ribulokinase[1], B0063[2][1], AraB[2][1], ribulokinase[2][1], ATP:L-ribulose 5-phosphotransferase[2][1] , ECK0064, JW0062, b0063

Product description

L-ribulokinase[2][3];

Component of L-ribulokinase[2][3]

Ribulokinase[4]

EC number (for enzymes)

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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0019568

arabinose catabolic process

PMID:13829634[5]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0008741

ribulokinase activity

PMID:13829634[5]

IMP: Inferred from Mutant Phenotype

F

complete

GO:0019569

L-arabinose catabolic process to xylulose 5-phosphate

ISA: Inferred from Sequence Alignment

P

E. coli AraB is xx% identical,

Missing: with/from, reference

GO:0000166

nucleotide binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0547

F

Seeded from EcoCyc (v14.0)

complete

GO:0005524

ATP binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0067

F

Seeded from EcoCyc (v14.0)

complete

GO:0008741

ribulokinase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_00520

F

Seeded from EcoCyc (v14.0)

complete

GO:0008741

ribulokinase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR005929

F

Seeded from EcoCyc (v14.0)

complete

GO:0008741

ribulokinase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:2.7.1.16

F

Seeded from EcoCyc (v14.0)

complete

GO:0008741

ribulokinase activity

PMID:5336963[6]

IDA: Inferred from Direct Assay

F

complete

GO:0005737

cytoplasm

PMID:5336963[6]

IDA: Inferred from Direct Assay

C

complete

GO:0005515

protein binding

PMID:5336963[6]

IDA: Inferred from Direct Assay

F

complete

GO:0016773

phosphotransferase activity, alcohol group as acceptor

PMID:5336963[6]

IDA: Inferred from Direct Assay

F

complete

GO:0019572

L-arabinose catabolic process

PMID:5336963[6]

IDA: Inferred from Direct Assay

P

complete

GO:0019568

arabinose catabolic process

PMID:5336963[6]

IDA: Inferred from Direct Assay

P

complete

GO:0016052

carbohydrate catabolic process

PMID:5336963[6]

IDA: Inferred from Direct Assay

P

complete

Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of L-ribulokinase

could be indirect

Protein

nadE

PMID:16606699[7]

Experiment(s):EBI-1135476

</protect>

Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes

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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc

MAIAIGLDFG SDSVRALAVD CATGEEIATS VEWYPRWQKG QFCDAPNNQF RHHPRDYIES
MEAALKTVLA ELSVEQRAAV VGIGVDSTGS TPAPIDADGN VLALRPEFAE NPNAMFVLWK
DHTAVEEAEE ITRLCHAPGN VDYSRYIGGI YSSEWFWAKI LHVTRQDSAV AQSAASWIEL
CDWVPALLSG TTRPQDIRRG RCSAGHKSLW HESWGGLPPA SFFDELDPIL NRHLPSPLFT
DTWTADIPVG TLCPEWAQRL GLPESVVISG GAFDCHMGAV GAGAQPNALV KVIGTSTCDI
LIADKQSVGE RAVKGICGQV DGSVVPGFIG LEAGQSAFGD IYAWFGRVLG WPLEQLAAQH
PELKTQINAS QKQLLPALTE AWAKNPSLDH LPVVLDWFNG RRTPNANQRL KGVITDLNLA
TDAPLLFGGL IAATAFGARA IMECFTDQGI AVNNVMALGG IARKNQVIMQ ACCDVLNRPL
QIVASDQCCA LGAAIFAAVA AKVHADIPSA QQKMASAVEK TLQPCSEQAQ RFEQLYRRYQ
QWAMSAEQHY LPTSAPAQAA QAVATL
Length

566

Mol. Wt

61.088 kDa

pI

5.2 (calculated)

Extinction coefficient

101,410 - 103,285 (calc based on 9 Y, 16 W, and 15 C residues)


Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P08204

Domain

3..193

PF00370 FGGY family of carbohydrate kinases, N-terminal domain

PMID:19920124[8]

Domain

289..501

PF02782 FGGY family of carbohydrate kinases, C-terminal domain

PMID:19920124[8]

<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=araB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:16128057

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:946017

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000223

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P08204

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG10053

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10053

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:946017

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000049

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0051

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. 1.0 1.1 1.2 1.3 1.4 1.5 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. 5.0 5.1 GROSS, J & ENGLESBERG, E (1959) Determination of the order of mutational sites governing L-arabinose utilization in Escherichia coli B/r bv transduction with phage Plbt. Virology 9 314-31 PubMed
  6. 6.0 6.1 6.2 6.3 6.4 6.5 6.6 Lee, N & Bendet, I (1967) Crystalline L-ribulokinase from Escherichia coli. J. Biol. Chem. 242 2043-50 PubMed
  7. Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. 8.0 8.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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