Tn3 tnpA:Gene Product(s)
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Function | Interactions | Localization | Sequence | Domains | Structure | Resources | Accessions | Links | References | Suggestions |
Nomenclature
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| Standard name |
Tn3 transposase |
|---|---|
| Synonyms |
TnpA |
| Product description |
Transposase[1] |
| EC number (for enzymes) |
|
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Notes
Function
Gene Ontology
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| Qualifier | GO ID | GO term name | Reference | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0000336 |
positive regulation of transposition, DNA-mediated |
IDA: Inferred from Direct Assay |
P |
Table 1 shows transposase activity in different solutions. In a heated solution, where transposase could not work properly, DNA binding was almost non-existent. |
complete | |||
| GO:0000345 |
cytosolic DNA-directed RNA polymerase complex |
IDA: Inferred from Direct Assay |
C |
Table 2 shows transposon binding to DNA at an RNA polymerase. |
complete | |||
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Interactions
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| Partner Type | Partner | Notes | References | Evidence |
|---|---|---|---|---|
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Notes
Localization
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| Compartment | Description | Evidence | Reference/Source | Notes |
|---|---|---|---|---|
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Notes
Structure and Physical Properties
Physical Properties
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| Name | |
|---|---|
| Sequence |
MLKKPSGREA DMPVDFLTTE QVESYGRFTG EPDELQLARY FHLDEADKEF IGKSRGDHNR LGIALQIGCV RFLGTFLTDM NHIPSGVRHF TARQLGIRDI TVLAEYGQRE NTRREHAALI RQHYQYREFA WPWTFRLTRL LYTRSWISNE RPGLLFDLAT GWLMQHRIIL PGATTLTRLI SEVREKATLR LWNKLALIPS AEQRSQLEML LGPTDCSRLS LLESLKKGPV TISGPAFNEA IERWKTLNDF GLHAENLSTL PAVRLKNLAR YAGMTSVFNI ARMSPQKRMA VLVAFVLAWE TLALDDALDV LDAMLAVIIR DARKIGQKKR LRSLKDLDKS ALALASACSY LLKEETPDES IRAEVFSYIP RQKLAEIITL VREIARPSDD NFHDEMVEQY GRVRRFLPHL LNTVKFSSAP AGVTTLNACD YLSREFSSRR QFFDDAPTEI ISQSWKRLVI NKEKHITRRG YTLCFLSKLQ DSLRRRDVYV TGSNRWGDPR ARLLQGADWQ ANRIKVYRSL GHPTDPQEAI KSLGHQLDSR YRQVAARLGE NEAVELDVSG PKPRLTISPL ASLDEPDSLK RLSKMISDLL PPVDLTELLL EINAHTGFAD EFFHASEASA RVDDLPVSIS AVLMAEACNI GLEPLIRSNV PALTRHRLNW TKANYLRAET ITSANARLVD FQATLPLAQI WGGGEVASAD GMRFVTPVRT INAGPNRKYF GNNRGITWYN FVSDQYSGFH GFHGIVIPGT LRDSIFVLEG LLEQETGLNP TEIMTDTAGA SDLVFGLFWL LGYQFSPRLA DAGASVFWRM DHDADYGVLN DIARGQSDPR KIVLQWDEMI RTAGSLKLGK VQASVLVRSL LKSERPSGLT QAIIEVGRIN KTLYLLNYID DEDYRRRILT QLNRGESRHA VARAICHGQK GEIRKRYTDG QEDQLGALGL VTNAVVLWNT IYMQAALDHL RAQGETLNDE DIARLSPLCH GHINMLGHYS FTLAELVTKG HLRPLKEASE AENVA |
| Length |
1,015 |
| Mol. Wt |
114.52 kDa |
| pI |
8.7 (calculated) |
| Extinction coefficient |
133,730 - 134,730 (calc based on 27 Y, 17 W, and 8 C residues) |
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Domains/Motifs/Modification Sites
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Structure<protect><protect> See Help:Product_structure for help entering or editing information in this section of EcoliWiki.
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Structure figures<protect> | |||
Notes
Gene Product Resources
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| Resource type | Source | Notes/Reference |
|---|---|---|
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Notes
Accessions in Other Databases
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See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
EcoCyc: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EcoGene: |
Escherichia coli str. K-12 substr. MG1655 | |
|
RegulonDB: |
Escherichia coli str. K-12 substr. MG1655 | |
|
EchoBASE: |
Escherichia coli str. K-12 substr. MG1655 | |
|
ASAP: |
Escherichia coli str. K-12 substr. MG1655 | |
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Notes
Links
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| Name | URL | Comments |
|---|---|---|
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References
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- ↑ Maekawa, T & Ohtsubo, E (1994) Identification of the region that determines the specificity of binding of the transposases encoded by Tn3 and gamma delta to the terminal inverted repeat sequences. Jpn. J. Genet. 69 269-85 PubMed
- ↑ Fennewald, MA et al. (1981) Purification of the Tn3 transposase and analysis of its binding to DNA. J. Biol. Chem. 256 4687-90 PubMed
- ↑ Wishart, WL et al. (1983) Escherichia coli RNA polymerase binding sites and transcription initiation sites in the transposon Tn3. Gene 24 99-113 PubMed
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