Plasmid F traI:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

traI

Synonyms

traI, Fpla107

Product description

traI

required for DNA transfer; nicks the DNA at oriT through N-terminal domain; C-terminal domain is a helicase that unwinds the DNA

EC number (for enzymes)


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Notes

Function

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<protect> Gene Ontology See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003678

DNA helicase activity

PMID:1651233[1]

IDA: Inferred from Direct Assay

F

complete

GO:0003697

single-stranded DNA binding

PMID:18611948[2]

IDA: Inferred from Direct Assay

F

See Figure 1.

complete

GO:0004003

ATP-dependent DNA helicase activity

PMID:2830275[3]

IDA: Inferred from Direct Assay

F

See Table III

complete

GO:0043141

ATP-dependent 5'-3' DNA helicase activity

PMID:2830275[3]

IDA: Inferred from Direct Assay

F

See Fig. 3

complete

GO:0017116

single-stranded DNA-dependent ATP-dependent DNA helicase activity

PMID:323494[4]

IDA: Inferred from Direct Assay

F

See Table 1 and Table 2.

complete

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Interactions <protect> See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

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Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence 
MMSIAQVRSA GSAGNYYTDK DNYYVLGSMG ERWAGRGAEQ LGLQGSVDKD VFTRLLEGRL
PDGADLSRMQ DGSNRHRPGY DLTFSAPKSV SMMAMLGGDK RLIDAHNQAV DFAVRQVEAL
ASTRVMTDGQ SETVLTGNLV MALFNHDTSR DQEPQLHTHA VVANVTQHNG EWKTLSSDKV
GKTGFIENVY ANQIAFGRLY REKLKEQVEA LGYETEVVGK HGMWEMPGVP VEAFSGRSQT
IREAVGEDAS LKSRDVAALD TRKSKQHVDP EIKMAEWMQT LKETGFDIRA YRDAADQRAD
LRTLTPGPAS QDGPDVQQAV TQAIAGLSER KVQFTYTDVL ARTVGILPPE NGVIERARAG
IDEAISREQL IPLDREKGLF TSGIHVLDEL SVRALSRDIM KQNRVTVHPE KSVPRTAGYS
DAVSVLAQDR PSLAIVSGQG GAAGQRERVA ELVMMAREQG REVQIIAADR RSQMNMKQDE
RLSGELITGR RQLLEGMAFT PGSTVIVDQG EKLSLKETLT LLDGAARHNV QVLITDSGQR
TGTGSALMAM KDAGVNTYRW QGGEQRPATI ISEPDRNVRY ARLAGDFAAS VKAGEESVAQ
VSGVREQAIL TQAIRSELKT QGVLGLPEVT MTALSPVWLD SRSRYLRDMY RPGMVMEQWN
PETRSHDRYV IDRVTAQSHS LTLRDAQGET QVVRISSLDS SWSLFRPEKM PVADGERLRV
TGKIPGLRVS GGDRLQVASV SEDAMTVVVP GRAEPATLPV SDSPFTALKL ENGWVETPGH
SVSDSATVFA SVTQMAMDNA TLNGLARSGR DVRLYSSLDE TRTAEKLARH PSFTVVSEQI
KTRAGETSLE TAISHQKSAL HTPAQQAIHL ALPVVESKKL AFSMVDLLTE AKSFAAEGTG
FTELGGEINA QIKRGDLLYV DVAKGYGTGL LVSRASYEAE KSILRHILEG KEAVMPLMER
VPGELMEKLT SGQRAATRMI LETSDRFTVV QGYAGVGKTT QFRAVMSAVN MLPESERPRV
VGLGPTHRAV GEMRSAGVDA QTLASFLHDT QLQQRSGETP DFSNTLFLLD ESSMVGNTDM
ARAYALIAAG GGRAVASGDT DQLQAIAPGQ PFRLQQTRSA ADVAIMKEIV RQTPELREAV
YSLINRDVER ALSGLESVKP SQVPRQEGAW APEHSVTEFS HSQEAKLAEA QQKAMLKGEA
FPDVPMTLYE AIVRDYTGRT PEAREQTLIV THLNEDRRVL NSMIHDVREK AGELGKEQVM
VPVLNTANIR DGELRRLSTW ETHRDALVLV DNVYHRIAGI SKDDGLITLQ DAEGNTRLIS
PREAVAEGVT LYTPDTIRVG TGDRMRFTKS DRERGYVANS VWTVTAVSGD SVTLSDGQQT
REIRPGQEQA EQHIDLAYAI TAHGAQGASE TFAIALEGTE GNRKLMAGFE SAYVALSRMK
QHVQVYTDNR QGWTDAINNA VQKGTAHDVF EPKPDREVMN AERLFSTARE LRDVAAGRAV
LRQAGLAGGD SPARFIAPGR KYPQPYVALP AFDRNGKSAG IWLNPLTTDD GNGLRGFSGE
GRVKGSGDAQ FVALQGSRNG ESLLADNMQD GVRIARDNPD SGVVVRIAGE GRPWNPGAIT
GGRVWGDIPD NSVQPGAGNG EPVTAEVLAQ RQAEEAIRRE TERRADEIVR KMAENKPDLP
DGKTEQAVRE IAGQERDRAA ITEREAALPE GVLREPQRVR EAVREIAREN LLQERLQQME
RDMVRDLQKE KTLGGD
Length

1,756

Mol. Wt

192.014 kDa

pI

6.0 (calculated)

Extinction coefficient

137,170 (calc based on 33 Y, 16 W, and 0 C residues)

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Domains/Motifs/Modification Sites

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Type Residues Description Notes References
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<motif_map/>

Structure
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Structures

<beststructure> gene=Plasmid_F_traI taxon=2465 </beststructure>

Models

View models at:

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</protect>

Structure figures

<protect>

</protect>

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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  1. ↑ Reygers, U et al. (1991) Endonuclease activity of Escherichia coli DNA helicase I directed against the transfer origin of the F factor. EMBO J. 10 2689-94 PubMed
  2. ↑ Hekman, K et al. (2008) An intrastrand three-DNA-base interaction is a key specificity determinant of F transfer initiation and of F TraI relaxase DNA recognition and cleavage. Nucleic Acids Res. 36 4565-72 PubMed
  3. ↑ 3.0 3.1 Lahue, EE & Matson, SW (1988) Escherichia coli DNA helicase I catalyzes a unidirectional and highly processive unwinding reaction. J. Biol. Chem. 263 3208-15 PubMed
  4. ↑ Abdel-Monem, M et al. (1977) Enzymatic unwinding of DNA. III. Mode of action of Escherichia coli DNA unwinding enzyme. J. Mol. Biol. 110 667-85 PubMed

Categories

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