Cis Element:Plasmid psi

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Cis element summary table

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Standard Name

psi

Synonyms
Function

Plasmid Recombination Site

Location
SO terms


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Notes

Location and Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

pSC101

GGTGCGCGCAA GATCCA TTATGTTAAAC[1][2]

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Notes

Interactions

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Partner Type Partner Notes References Evidence

Protein

XerC

could be indirect

plasmid stability assays

Protein

XerD

could be indirect

plasmid stability assays

Protein

PepA

PMID:8605888[3]


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Notes

psi can promote intermolecular recombination and replace dif on the chromosome[2]. The product of recombination at psi is a right-handed antiparallel catenane[4].

Mutant alleles and phenotypes

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Allele Nt change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

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Notes

Accessions

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Database Accession Notes

EcoCyc

EcoCyc:

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:

Escherichia coli str. K-12 substr. MG1655

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Notes

References

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  1. Kuempel, PL et al. (1991) dif, a recA-independent recombination site in the terminus region of the chromosome of Escherichia coli. New Biol. 3 799-811 PubMed
  2. 2.0 2.1 2.2 2.3 Cornet, F et al. (1994) Plasmid pSC101 harbors a recombination site, psi, which is able to resolve plasmid multimers and to substitute for the analogous chromosomal Escherichia coli site dif. J. Bacteriol. 176 3188-95 PubMed
  3. 3.0 3.1 3.2 Colloms, SD et al. (1996) Xer-mediated site-specific recombination in vitro. EMBO J. 15 1172-81 PubMed
  4. Colloms, SD et al. (1997) Topological selectivity in Xer site-specific recombination. Cell 88 855-64 PubMed

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