Category:Strain:S26 R1d
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| Name |
S26R1d |
|---|---|
| Synonyms | |
| Genotype |
Hfr (PO2A) fhuA22 phoA4(Am) glnV33(AS) ompF627(T2R) fadL701(T2R) relA1 pitA10 spoT1 rrnB-2 mcrB1 creC510 |
| Phenotype |
phosphatase positive |
| Plasmids |
Hfr |
| Phage |
λ+ |
| Sources | |
| Construction |
Strain S26R1d is a spontaneous isolate of strain S26 |
| References |
PMID:13896933[1] PMID:5327650[2] PMID:4568763[3] PMID:4564480[4] PMID:4286570[5] |
Notes
This strain carries the S26 amber mutation (TAG) in the phosphatase gene.
This strain carries the supE suppressor (inserts glutamine at the amber position) and is therefore capable of suppressing the S26 phosphatase nonsense mutation, as well as amber mutations in other genes. However, the efficiency of suppression may vary considerably depending on the sequence context of the amber codon.
References
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- ↑ GAREN, A & SIDDIQI, O (1962) Suppression of mutations in the alkaline phosphatase structural cistron of E. coli. Proc. Natl. Acad. Sci. U.S.A. 48 1121-7 PubMed
- ↑ Garen, A et al. (1965) Suppressor genes for nonsense mutations. I. The Su-1, Su-2 and Su-3 genes of Escherichia coli. J. Mol. Biol. 14 167-78 PubMed
- ↑ Bachmann, BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36 525-57 PubMed
- ↑ Remaut, E & Fiers, W (1972) Studies on the bacteriophage MS2. XVI. The termination signal of the A protein cistron. J. Mol. Biol. 71 243-61 PubMed
- ↑ Weigert, MG et al. (1965) Amino acid substitutions resulting from suppression of nonsense mutations. II. Glutamine insertion by the Su-2 gene; tyrosine insertion by the Su-3 gene. J. Mol. Biol. 14 522-7 PubMed
