htpX:Gene Product(s)

Standard name	HtpX
Synonyms	predicted endopeptidase^[1], B1829^[2]^[1], HtpX^[2]^[1]
Product description	heat shock protein, integral membrane protein^[2]^[3] Zinc metalloprotease HtpX, membrane-bound; cleaves SecY in vivo and in vitro; self-cleaves; M48 family^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0004222	metalloendopeptidase activity	GO_REF:0000002 GO_REF:0000002 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR001915 InterPro:IPR009481 HAMAP:MF_00188	F	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0006508	proteolysis	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001915	P	Seeded from EcoCyc ^[6]	complete
	GO:0006950	response to stress	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P	Seeded from EcoCyc ^[6]	complete
	GO:0008233	peptidase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0645	F	Seeded from EcoCyc ^[6]	complete
	GO:0008237	metallopeptidase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0482	F	Seeded from EcoCyc ^[6]	complete
	GO:0008270	zinc ion binding	GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR009481 SP_KW:KW-0862 HAMAP:MF_00188	F	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0016020	membrane	GO_REF:0000002 GO_REF:0000004 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001915 SP_KW:KW-0397 SP_KW:KW-0472	C	Seeded from EcoCyc 12.5 ^[5]	complete
	GO:0016021	integral to membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc ^[6]	complete
	GO:0016787	hydrolase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc ^[6]	complete
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc ^[6]	complete
	GO:0009266	response to temperature stimulus				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0005886	plasma membrane	GO_REF:0000020 GO_REF:0000004 GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	HAMAP:MF_00188 SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037	C	Seeded from EcoCyc ^[6]	complete
	GO:0004222	metalloendopeptidase activity	GO_REF:0000020 GO_REF:0000002 GO_REF:0000002 PMID:16076848^[7]	IDA: Inferred from Direct Assay	HAMAP:MF_00188 InterPro:IPR001915 InterPro:IPR009481	F	Seeded from EcoCyc	complete
	GO:0005886	plasma membrane	GO_REF:0000020 GO_REF:0000004 GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	HAMAP:MF_00188 SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037	C	Seeded from EcoCyc	complete
	GO:0006508	proteolysis	GO_REF:0000002	IEA: Inferred from Electronic Annotation	InterPro:IPR001915	P	Seeded from EcoCyc	complete
	GO:0006950	response to stress	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0346	P	Seeded from EcoCyc	complete
	GO:0008233	peptidase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0645	F	Seeded from EcoCyc	complete
	GO:0008237	metallopeptidase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0482	F	Seeded from EcoCyc	complete
	GO:0008270	zinc ion binding	GO_REF:0000020 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	HAMAP:MF_00188 InterPro:IPR009481 SP_KW:KW-0862	F	Seeded from EcoCyc	complete
	GO:0016020	membrane	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR001915 SP_KW:KW-0472	C	Seeded from EcoCyc	complete
	GO:0016021	integral to membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc	complete
	GO:0016787	hydrolase activity	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0378	F	Seeded from EcoCyc	complete
	GO:0046872	metal ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0479	F	Seeded from EcoCyc	complete
	GO:0004222	metalloendopeptidase activity	PMID:16076848^[7]	IDA: Inferred from Direct Assay		F		complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

HtpX is ATP-independent and introduces endoproteolytic cuts in abnormal membrane proteins

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence
plasma membrane	C-terminus localized in the cytoplasm with 4 predicted transmembrane domains	Daley et al. (2005) ^[8]
plasma membrane
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MMRIALFLLT NLAVMVVFGL VLSLTGIQSS SVQGLMIMAL LFGFGGSFVS LLMSKWMALR SVGGEVIEQP RNERERWLVN TVATQARQAG IAMPQVAIYH APDINAFATG ARRDASLVAV STGLLQNMSP DEAEAVIAHE ISHIANGDMV TMTLIQGVVN TFVIFISRIL AQLAAGFMGG NRDEGEESNG NPLIYFAVAT VLELVFGILA SIITMWFSRH REFHADAGSA KLVGREKMIA ALQRLKTSYE PQEATSMMAL CINGKSKSLS ELFMTHPPLD KRIEALRTGE YLK
Length	293
Mol. Wt	31.923 kDa
pI	7.1 (calculated)
Extinction coefficient	22,460 - 22,585 (calc based on 4 Y, 3 W, and 1 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes
Domain	32 - 54	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	158 - 180	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	195 - 217	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	5 - 27	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

Models

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:EG10462-MONOMER
UniProt (EcoliWiki Page)	UniProt:P23894
PFAM (EcoliWiki Page)	PFAM:PF06509
RefSeq (EcoliWiki Page)	RefSeq:NP_416343
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^6.0 ^6.1 ^6.2 ^6.3 ^6.4 ^6.5 ^6.6 ^6.7 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ ^7.0 ^7.1 Sakoh, M et al. (2005) Proteolytic activity of HtpX, a membrane-bound and stress-controlled protease from Escherichia coli. J. Biol. Chem. 280 33305-10 PubMed
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ ^9.0 ^9.1 ^9.2 ^9.3 Krogh, A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 567-80 PubMed

htpX:Gene Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

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htpX:Gene Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Structure figures

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

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