mdtK:Gene Product(s)

Standard name	MdtK
Synonyms	multidrug efflux system transporter^[1], NorM^[2]^[1], B1663^[2]^[1], MdtK^[2]^[1], YdhE^[2]^[1]
Product description	NorE multidrug efflux MATE transporter^[2]^[3] Multidrug resistance efflux protein, 12 transmembrane helices; overexpression resistance to norfloxacin, TTP, deoxycholate, trimethoprim^[4]
EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier	GO ID	GO term name	Reference(s)	Evidence Code	with/from	Aspect	Notes	Status
	GO:0006810	transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc ^[5]	complete
	GO:0006811	ion transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc ^[5]	complete
	GO:0006814	sodium ion transport	GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	SP_KW:KW-0739 HAMAP:MF_00400	P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0006855	multidrug transport	GO_REF:0000002 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002528 HAMAP:MF_00400	P	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0015238	drug transporter activity	GO_REF:0000002 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002528 HAMAP:MF_00400	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0015297	antiporter activity	GO_REF:0000002 GO_REF:0000004 GO_REF:0000020	IEA: Inferred from Electronic Annotation	InterPro:IPR002528 SP_KW:KW-0050 HAMAP:MF_00400	F	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0016020	membrane	GO_REF:0000002 GO_REF:0000004 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR002528 SP_KW:KW-0397 SP_KW:KW-0472	C	Seeded from EcoCyc 12.5 ^[6]	complete
	GO:0016021	integral to membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc ^[5]	complete
	GO:0031402	sodium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0915	F	Seeded from EcoCyc ^[5]	complete
	GO:0046677	response to antibiotic	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc ^[5]	complete
	GO:0042493	response to drug				P	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0005886	plasma membrane	GO_REF:0000020 GO_REF:0000004 GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	HAMAP:MF_00400 SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037	C	Seeded from EcoCyc ^[5]	complete
	GO:0005886	plasma membrane	GO_REF:0000020 GO_REF:0000004 GO_REF:0000004 GO_REF:0000023	IEA: Inferred from Electronic Annotation	HAMAP:MF_00400 SP_KW:KW-0997 SP_KW:KW-1003 SP_SL:SL-0037	C	Seeded from EcoCyc	complete
	GO:0006810	transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0813	P	Seeded from EcoCyc	complete
	GO:0006811	ion transport	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0406	P	Seeded from EcoCyc	complete
	GO:0006814	sodium ion transport	GO_REF:0000020 GO_REF:0000004	IEA: Inferred from Electronic Annotation	HAMAP:MF_00400 SP_KW:KW-0739	P	Seeded from EcoCyc	complete
	GO:0006855	multidrug transport	GO_REF:0000020 GO_REF:0000002	IEA: Inferred from Electronic Annotation	HAMAP:MF_00400 InterPro:IPR002528	P	Seeded from EcoCyc	complete
	GO:0015238	drug transporter activity	GO_REF:0000020 GO_REF:0000002	IEA: Inferred from Electronic Annotation	HAMAP:MF_00400 InterPro:IPR002528	F	Seeded from EcoCyc	complete
	GO:0015297	antiporter activity	GO_REF:0000020 GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	HAMAP:MF_00400 InterPro:IPR002528 SP_KW:KW-0050	F	Seeded from EcoCyc	complete
	GO:0015559	multidrug efflux pump activity	PMID:18591276^[7]	IDA: Inferred from Direct Assay		F	Seeded from EcoCyc	complete
	GO:0016020	membrane	GO_REF:0000002 GO_REF:0000004	IEA: Inferred from Electronic Annotation	InterPro:IPR002528 SP_KW:KW-0472	C	Seeded from EcoCyc	complete
	GO:0016021	integral to membrane	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0812	C	Seeded from EcoCyc	complete
	GO:0031402	sodium ion binding	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0915	F	Seeded from EcoCyc	complete
	GO:0046677	response to antibiotic	GO_REF:0000004	IEA: Inferred from Electronic Annotation	SP_KW:KW-0046	P	Seeded from EcoCyc	complete
	GO:0015292	uniporter activity				F	Seeded from EcoCyc 11.1^[3].	required fields missing
	GO:0015559	multidrug efflux pump activity	PMID:18591276^[7]	IDA: Inferred from Direct Assay		F	Decreased accumulation of norfloxacin and ethidium bromide compared to vector control.	complete
	GO:0015559	multidrug efflux pump activity	PMID:18591276^[7]	ISA: Inferred from Sequence Alignment	GenBank:YP_207551	F	NorM from Neisseria gonorrhea	complete
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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type	Partner	Notes	References	Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment	Description	Evidence	Notes
plasma membrane	C-terminus localized in the cytoplasm with 12 predicted transmembrane domains	Daley et al. (2005) ^[8]
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence	at EcoCyc MQKYISEARL LLALAIPVIL AQIAQTAMGF VDTVMAGGYS ATDMAAVAIG TSIWLPAILF GHGLLLALTP VIAQLNGSGR RERIAHQVRQ GFWLAGFVSV LIMLVLWNAG YIIRSMENID PALADKAVGY LRALLWGAPG YLFFQVARNQ CEGLAKTKPG MVMGFIGLLV NIPVNYIFIY GHFGMPELGG VGCGVATAAV YWVMFLAMVS YIKRARSMRD IRNEKGTAKP DPAVMKRLIQ LGLPIALALF FEVTLFAVVA LLVSPLGIVD VAGHQIALNF SSLMFVLPMS LAAAVTIRVG YRLGQGSTLD AQTAARTGLM VGVCMATLTA IFTVSLREQI ALLYNDNPEV VTLAAHLMLL AAVYQISDSI QVIGSGILRG YKDTRSIFYI TFTAYWVLGL PSGYILALTD LVVEPMGPAG FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI ILQRASR
Length	457
Mol. Wt	49.448 kDa
pI	10.0 (calculated)
Extinction coefficient	62,340 - 62,715 (calc based on 16 Y, 7 W, and 3 C residues)
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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type	Residues	Description	Notes
Domain	241 - 263	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	278 - 300	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	313 - 335	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	350 - 367	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	387 - 409	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	419 - 438	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	7 - 29	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	44 - 66	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	91 - 113	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	128 - 147	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	160 - 180	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
Domain	190 - 212	Predicted membrane-spanning helix.	TMD predicted by TMHMM^[9]
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<motif_map/>

Click the image to view raw output from TMHMM.

Structure See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

Models

View models at:

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type	Source	Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database	Accession	Notes
EcoCyc (EcoliWiki Page)	EcoCyc:YDHE-MONOMER
UniProt (EcoliWiki Page)	UniProt:P37340
PFAM (EcoliWiki Page)	PFAM:PF01554
RefSeq (EcoliWiki Page)	RefSeq:YP_025307
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Notes

Links

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Name	URL	Comments
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References

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See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 ^1.2 ^1.3 ^1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
↑ ^2.0 ^2.1 ^2.2 ^2.3 ^2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^3.0 ^3.1 ^3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
↑ ^5.0 ^5.1 ^5.2 ^5.3 ^5.4 ^5.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
↑ ^6.0 ^6.1 ^6.2 ^6.3 ^6.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
↑ ^7.0 ^7.1 ^7.2 Long, F et al. (2008) Functional cloning and characterization of the multidrug efflux pumps NorM from Neisseria gonorrhoeae and YdhE from Escherichia coli. Antimicrob. Agents Chemother. 52 3052-60 PubMed
↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
↑ ^9.00 ^9.01 ^9.02 ^9.03 ^9.04 ^9.05 ^9.06 ^9.07 ^9.08 ^9.09 ^9.10 ^9.11 Krogh, A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 567-80 PubMed

mdtK:Gene Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

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mdtK:Gene Product(s)

Nomenclature

Notes

Function

Notes

Localization

Notes

Structure and Physical Properties

Notes

Gene Product Resources

Notes

Accessions in Other Databases

Notes

Links

References

Categories

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