Phage MS2
Bacteriophage MS2 [NC_001417]
Morphology
Genome Organization
3569 bases of ssRNA, linear, (+) strand
4 genes encoded; gene order: A (maturation), coat, lysis (overlaps both coat and rep genes), rep (replicase)
Life cycle
Adsorption and Injection
Absorbs to F pilus of E. coli
RNA replication
- Requires the MS2-encoded protein, Replicase, as well as 3 host proteins: EF-Ts, EF-Tu & ribosomal protein S1
- Produces (-) strands that are complementary to the packaged strand and (+) strands, which serve as the template mRNA for translation of the MS2 proteins & is also packaged
- Replicase is extremely error-prone relative to DNA replication machinery
- MS2 does NOT require Hfq, unlike Qbeta
Packaging
- T=3 morphology, with 180 copies of coat and addition of a single copy of A protein
- Packaging of RNA genome is proposed to begin with aggregation of coat protein at specific regions of the genome
Lysis
- The L protein is necessary and sufficient to elicit lysis of E. coli
See also
References
See Help:References for how to manage references in EcoliWiki.