BJ5183

Alternate Names

BJ5183 is a component of Strategene's AdEasy system for construction of Adenovirus vectors.

Sources

Chromosomal Genotype

This genotype is inferred from the description in Takahashi et al. ^[1] as:

recB recC sbcB sbcC endA galK met thi-1 bioT hsdR rpsL(strR)

The nature of bioT is unclear, as this is not a listed synonym for any annotated E. coli gene.

Plasmids

not specified

Phage

not specified

Phenotypes

Construction

unknown parents.

Notes

BJ5183 is a recBC sbc strain used for doing homologous recombination with linear dsDNA fragments. The strain originates with D. Hanahan, but it's construction is not well documented. The recombination properties of BJ5183 have been examined by Takahashi et al. ^[1].

References

See Help:References for how to manage references in EcoliWiki.

↑ ^1.0 ^1.1 Takahashi, N et al. (2003) An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks. Gene 303 89-97 PubMed

[PMID:12559570-1] 1.0 ^1.1 Takahashi, N et al. (2003) An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks. Gene 303 89-97 PubMed

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