Many Microbe Microarrays Database (M3D)

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Many Microbe Microarrays Database (M3D)
Who: Tim Gardner http://gardnerlab.bu.edu/
What: E. coli Affymetrix data
Where: Boston University Many Microbe Microarrays Database (M3D)
Updates
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Web Services

About Many Microbe Microarrays Database (M3D)

Many Microbe Microarrays Database (M3D) is a resource for analyzing and retrieving gene expression data for microbes. The database currently contains affymetrix expression profiles for Escherichia coli. In the future, Shewanella oneidensis affymetrix data will be available as well.

Content

Experiments: 189 Arrays: 445

Using Many Microbe Microarrays Database (M3D)

Browsing

Searching

Users can query M3D by choosing a project and a list of genes. Data can be normalized (scaling by gene) prior to analysis.

http://m3d.bu.edu/cgi-bin/web/array/index.pl?db=E_coli_v3_Build_1

Presentation formats include:

  • Heat Plot (genes vs experiments)
  • Histogram of individual gene expression (one plot per gene)
  • Histogram of all gene expression (one plot for N genes)
  • Plot Expression of many genes as a function of one gene
  • Link to download expression data

Average Array Replicates by:

  • RMA
  • GCRMA
  • DchipPM
  • DchipMM
  • MAS5

Heat Plot Parameters:

  • rescale data to within stddev of mean
  • Order Genes By Correlation
  • Order Experiments By Correlation

Heat Map coloring:

  • Red/Blue
  • Red/Green


M3D provides a meatalink to execute queries on N-browse, which is described as an interactive graphical browser for molecular interaction networks

http://nematoda.bio.nyu.edu:8080/NBrowse/N-Browse.jsp?last=false

Usage examples

Add links to additional pages describing success stories here.

Other sites with related content

Technology

The following free software and libraries were used for array normalization and to develop M3D: dChip, Bioconductor (for GCRMA and RMA), gd, cgic, Cluster 3.0, and MySQL.

Web Services/API

Discussion

References

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External Links

Many Microbe Microarrays Database (M3D)

Discussion of Many Microbe Microarrays Database (M3D) on other websites

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