DH5 alpha

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Alternate Names

Sources

Chromosomal Genotype

fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17

Plasmids

Phage

Phenotypes

Construction

Notes

Transforms with high efficiency. Like many cloning strains, DH5 alpha has several features that make it useful for recombinant DNA methods.

  • The endA1 mutation inactivates an intracellular endonuclease that degrades plasmid DNA in many miniprep methods[1].
  • The hsdR17 mutation eliminates the restriction endonuclease of the EcoKI restriction-modification system, so DNA lacking the EcoKI methylation will not be degraded.
  • Δ(lacZ)M15 is the alpha acceptor allele needed for blue-white screening with many lacZ based vectors.
  • recA eliminates homologous recombination. This makes the strain somewhat sickly, but reduces deletion formation and plasmid multimerization[2].

References

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  1. ↑ Taylor, RG et al. (1993) E. coli host strains significantly affect the quality of small scale plasmid DNA preparations used for sequencing. Nucleic Acids Res. 21 1677-8 PubMed
  2. ↑ citation needed
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