mdtK:Gene Product(s)

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

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Standard name

MdtK

Synonyms

multidrug efflux system transporter[1], NorM[2][1], B1663[2][1], MdtK[2][1], YdhE[2][1]

Product description

NorE multidrug efflux MATE transporter[2][3]

Multidrug resistance efflux protein, 12 transmembrane helices; overexpression resistance to norfloxacin, TTP, deoxycholate, trimethoprim[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0015559

multidrug efflux pump activity

PMID:18591276[5]

IDA: Inferred from Direct Assay

1

Decreased accumulation of norfloxacin and ethidium bromide compared to vector control.

complete

GO:0015559

multidrug efflux pump activity

PMID:18591276[5]

ISA: Inferred from Sequence Alignment

GenBank:YP_207551

1

NorM from Neisseria gonorrhea

complete

GO:0005215

transporter activity

PMID:18591276[5]

IDA: Inferred from Direct Assay

F

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [6]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MQKYISEARL LLALAIPVIL AQIAQTAMGF VDTVMAGGYS ATDMAAVAIG TSIWLPAILF
GHGLLLALTP VIAQLNGSGR RERIAHQVRQ GFWLAGFVSV LIMLVLWNAG YIIRSMENID
PALADKAVGY LRALLWGAPG YLFFQVARNQ CEGLAKTKPG MVMGFIGLLV NIPVNYIFIY
GHFGMPELGG VGCGVATAAV YWVMFLAMVS YIKRARSMRD IRNEKGTAKP DPAVMKRLIQ
LGLPIALALF FEVTLFAVVA LLVSPLGIVD VAGHQIALNF SSLMFVLPMS LAAAVTIRVG
YRLGQGSTLD AQTAARTGLM VGVCMATLTA IFTVSLREQI ALLYNDNPEV VTLAAHLMLL
AAVYQISDSI QVIGSGILRG YKDTRSIFYI TFTAYWVLGL PSGYILALTD LVVEPMGPAG
FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI ILQRASR
Length

457

Mol. Wt

49.448 kDa

pI

10.0 (calculated)

Extinction coefficient

62,340 - 62,715 (calc based on 16 Y, 7 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Domain

17..177

PF01554 MatE

PMID:19920124[7]

Domain

244..405

PF01554 MatE

PMID:19920124[7]

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<motif_map/>

tmhmm.php?gene=mdtK&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mdtK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:YDHE-MONOMER

UniProt (EcoliWiki Page)

UniProt:P37340

PFAM (EcoliWiki Page)

Pfam:P37340

RefSeq (EcoliWiki Page)

RefSeq:YP_025307


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 Long, F et al. (2008) Functional cloning and characterization of the multidrug efflux pumps NorM from Neisseria gonorrhoeae and YdhE from Escherichia coli. Antimicrob. Agents Chemother. 52 3052-60 PubMed
  6. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  7. ↑ 7.0 7.1 Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

Categories

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