mdtK:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Standard name
!align=left |Standard name
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MdtK  
MdtK  
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!align=left {{table heading style}} |Synonyms
!align=left |Synonyms
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multidrug efflux system transporter<ref name='LIB:Riley_2006'/>, NorM<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, B1663<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MdtK<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YdhE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
multidrug efflux system transporter<ref name='LIB:Riley_2006'/>, NorM<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, B1663<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MdtK<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YdhE<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
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!align=left {{table heading style}} |Product description
!align=left |Product description
||
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NorE multidrug efflux MATE transporter<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
NorE multidrug efflux MATE transporter<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>
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Multidrug resistance efflux protein, 12 transmembrane helices; overexpression resistance to norfloxacin, TTP, deoxycholate, trimethoprim<ref name='LIB:EcoGene'/>
Multidrug resistance efflux protein, 12 transmembrane helices; overexpression resistance to norfloxacin, TTP, deoxycholate, trimethoprim<ref name='LIB:EcoGene'/>
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!align=left {{table heading style}} |EC number (for enzymes)
!align=left |EC number (for enzymes)
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!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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complete  
complete  


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!align=left {{table heading style}} |Sequence
!align=left |Sequence
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[http://biocyc.org/ECOLI/sequence-aa?object=YDHE-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=YDHE-MONOMER at EcoCyc]  
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FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI ILQRASR</pre>  
FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI ILQRASR</pre>  
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!align=left {{table heading style}} |Length
!align=left |Length
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457
457
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!align=left {{table heading style}} |Mol. Wt
!align=left |Mol. Wt
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49.448 kDa
49.448 kDa
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!align=left {{table heading style}} |pI
!align=left |pI
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10.0 (calculated)
10.0 (calculated)
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!align=left {{table heading style}} |Extinction coefficient
!align=left |Extinction coefficient
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62,340 - 62,715 (calc based on 16 Y, 7 W, and 3 C residues)
62,340 - 62,715 (calc based on 16 Y, 7 W, and 3 C residues)


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<beststructure>
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</beststructure>
</beststructure>
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View models at:
View models at:
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* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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Revision as of 02:06, 9 January 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

MdtK

Synonyms

multidrug efflux system transporter[1], NorM[2][1], B1663[2][1], MdtK[2][1], YdhE[2][1]

Product description

NorE multidrug efflux MATE transporter[2][3]

Multidrug resistance efflux protein, 12 transmembrane helices; overexpression resistance to norfloxacin, TTP, deoxycholate, trimethoprim[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0006810

transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc [5]

complete

GO:0006811

ion transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0406

P

Seeded from EcoCyc [5]

complete

GO:0006814

sodium ion transport

GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

SP_KW:KW-0739
HAMAP:MF_00400

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0006855

multidrug transport

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002528
HAMAP:MF_00400

P

Seeded from EcoCyc 12.5 [6]

complete

GO:0015238

drug transporter activity

GO_REF:0000002
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002528
HAMAP:MF_00400

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0015297

antiporter activity

GO_REF:0000002
GO_REF:0000004
GO_REF:0000020

IEA: Inferred from Electronic Annotation

InterPro:IPR002528
SP_KW:KW-0050
HAMAP:MF_00400

F

Seeded from EcoCyc 12.5 [6]

complete

GO:0016020

membrane

GO_REF:0000002
GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR002528
SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [6]

complete

GO:0016021

integral to membrane

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0812

C

Seeded from EcoCyc [5]

complete

GO:0031402

sodium ion binding

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0915

F

Seeded from EcoCyc [5]

complete

GO:0046677

response to antibiotic

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0046

P

Seeded from EcoCyc [5]

complete

GO:0042493

response to drug

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005886

plasma membrane

GO_REF:0000020
GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

HAMAP:MF_00400
SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [5]

complete

GO:0015292

uniporter activity

F

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0015559

multidrug efflux pump activity

PMID:18591276[7]

IDA: Inferred from Direct Assay

F

Decreased accumulation of norfloxacin and ethidium bromide compared to vector control.

complete

GO:0015559

multidrug efflux pump activity

PMID:18591276[7]

ISA: Inferred from Sequence Alignment

GenBank:YP_207551

F

NorM from Neisseria gonorrhea

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes

plasma membrane

C-terminus localized in the cytoplasm with 12 predicted transmembrane domains

Daley et al. (2005) [8]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MQKYISEARL LLALAIPVIL AQIAQTAMGF VDTVMAGGYS ATDMAAVAIG TSIWLPAILF
GHGLLLALTP VIAQLNGSGR RERIAHQVRQ GFWLAGFVSV LIMLVLWNAG YIIRSMENID
PALADKAVGY LRALLWGAPG YLFFQVARNQ CEGLAKTKPG MVMGFIGLLV NIPVNYIFIY
GHFGMPELGG VGCGVATAAV YWVMFLAMVS YIKRARSMRD IRNEKGTAKP DPAVMKRLIQ
LGLPIALALF FEVTLFAVVA LLVSPLGIVD VAGHQIALNF SSLMFVLPMS LAAAVTIRVG
YRLGQGSTLD AQTAARTGLM VGVCMATLTA IFTVSLREQI ALLYNDNPEV VTLAAHLMLL
AAVYQISDSI QVIGSGILRG YKDTRSIFYI TFTAYWVLGL PSGYILALTD LVVEPMGPAG
FWIGFIIGLT SAAIMMMLRM RFLQRLPSAI ILQRASR
Length

457

Mol. Wt

49.448 kDa

pI

10.0 (calculated)

Extinction coefficient

62,340 - 62,715 (calc based on 16 Y, 7 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes
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<motif_map/>

tmhmm.php?gene=mdtK&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=mdtK taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:YDHE-MONOMER

UniProt (EcoliWiki Page)

UniProt:P37340

PFAM (EcoliWiki Page)

PFAM:PF01554

RefSeq (EcoliWiki Page)

RefSeq:YP_025307


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  6. ↑ 6.0 6.1 6.2 6.3 6.4 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  7. ↑ 7.0 7.1 Long, F et al. (2008) Functional cloning and characterization of the multidrug efflux pumps NorM from Neisseria gonorrhoeae and YdhE from Escherichia coli. Antimicrob. Agents Chemother. 52 3052-60 PubMed
  8. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

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