ispB:Gene Product(s): Difference between revisions

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!align=left {{table heading style}} |Standard name
!align=left |Standard name
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IspB  
IspB  
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!align=left {{table heading style}} |Synonyms
!align=left |Synonyms
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octaprenyl diphosphate synthase<ref name='LIB:Riley_2006'/>, B3187<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cel<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhbD<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, IspB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
octaprenyl diphosphate synthase<ref name='LIB:Riley_2006'/>, B3187<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cel<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhbD<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, IspB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
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!align=left {{table heading style}} |Product description
!align=left |Product description
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OPPSYN<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
OPPSYN<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
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Octaprenyl diphosphate synthase, isoprenoid biosynthesis<ref name='LIB:EcoGene'/>
Octaprenyl diphosphate synthase, isoprenoid biosynthesis<ref name='LIB:EcoGene'/>
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!align=left {{table heading style}} |EC number (for enzymes)
!align=left |EC number (for enzymes)
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* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.- 2.5.1.-]<ref name='LIB:Riley_2006'/>
* [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.- 2.5.1.-]<ref name='LIB:Riley_2006'/>


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!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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Experiment(s):EBI-1145102<br />  
Experiment(s):EBI-1145102<br />  


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!|Compartment!!Description!!Evidence!!Source!!Notes
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|-
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!align=left {{table heading style}} |Sequence
!align=left |Sequence
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[http://biocyc.org/ECOLI/sequence-aa?object=OPPSYN-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=OPPSYN-MONOMER at EcoCyc]  
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VLPDTPWREA LIGLAHIAVQ RDR</pre>  
VLPDTPWREA LIGLAHIAVQ RDR</pre>  
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!align=left {{table heading style}} |Length
!align=left |Length
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323
323
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!align=left {{table heading style}} |Mol. Wt
!align=left |Mol. Wt
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35.217 kDa
35.217 kDa
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!align=left {{table heading style}} |pI
!align=left |pI
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4.6 (calculated)
4.6 (calculated)
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!align=left {{table heading style}} |Extinction coefficient
!align=left |Extinction coefficient
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24,410 - 24,785 (calc based on 9 Y, 2 W, and 3 C residues)
24,410 - 24,785 (calc based on 9 Y, 2 W, and 3 C residues)


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<beststructure>
<beststructure>
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</beststructure>
</beststructure>
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View models at:
View models at:
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* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)
* [http://spock.genes.nig.ac.jp/~genome/search.html Genomes TO Protein] (GTOP)


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Revision as of 01:50, 9 January 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

IspB

Synonyms

octaprenyl diphosphate synthase[1], B3187[2][1], Cel[2][1], YhbD[2][1], IspB[2][1]

Product description

OPPSYN[2][3];

Component of octaprenyl diphosphate synthase[3]

Octaprenyl diphosphate synthase, isoprenoid biosynthesis[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0004659

prenyltransferase activity

PMID:11825609[5]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc [6]

complete

GO:0005515

protein binding

PMID:16606699[7]

IPI: Inferred from Physical Interaction

UniProtKB:P60422

F

Seeded from EcoCyc [6]

complete

GO:0016094

polyprenol biosynthetic process

PMID:11108713[8]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc [6]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0008299

isoprenoid biosynthetic process

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000092
SP_KW:KW-0414

P

Seeded from EcoCyc [6]

complete

GO:0016740

transferase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0808

F

Seeded from EcoCyc [6]

complete

GO:0006744

ubiquinone biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of octaprenyl diphosphate synthase

could be indirect

Protein

rplB

PMID:16606699[7]

Experiment(s):EBI-1145102
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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM IAVLAARAVG
YEGNAHVTIA ALIEFIHTAT LLHDDVVDES DMRRGKATAN AAFGNAASVL VGDFIYTRAF
QMMTSLGSLK VLEVMSEAVN VIAEGEVLQL MNVNDPDITE ENYMRVIYSK TARLFEAAAQ
CSGILAGCTP EEEKGLQDYG RYLGTAFQLI DDLLDYNADG EQLGKNVGDD LNEGKPTLPL
LHAMHHGTPE QAQMIRTAIE QGNGRHLLEP VLEAMNACGS LEWTRQRAEE EADKAIAALQ
VLPDTPWREA LIGLAHIAVQ RDR
Length

323

Mol. Wt

35.217 kDa

pI

4.6 (calculated)

Extinction coefficient

24,410 - 24,785 (calc based on 9 Y, 2 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=ispB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:OPPSYN-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AD57

PFAM (EcoliWiki Page)

PFAM:PF00348

RefSeq (EcoliWiki Page)

RefSeq:NP_417654


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Pan, JJ et al. (2002) Insight into the activation mechanism of Escherichia coli octaprenyl pyrophosphate synthase derived from pre-steady-state kinetic analysis. Biochim Biophys Acta 1594 64-73 PubMed
  6. ↑ 6.0 6.1 6.2 6.3 6.4 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. ↑ 7.0 7.1 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  8. ↑ Kainou, T et al. (2001) Dimer formation of octaprenyl-diphosphate synthase (IspB) is essential for chain length determination of ubiquinone. J Biol Chem 276 7876-83 PubMed

Categories

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