nrdA:Quickview: Difference between revisions
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Reducing equivalents provided by glutaredoxin or thioredoxin. Binds TrxA (Kumar, 2004). About half of the overproduced protein has the first one or two amino acids removed. E. coli B native protein is reported to start with Gln. a second native E. coli B protein start may correspond to a cleavage after Leu26. Leu26 CTG codon is unlikely to be an alternative start codon in K-12 or B and no evidence of this cleavage or an alternative start codon was seen with the overproduced K-12 protein. An M-N or MN-Q cleavage by MAP would be unusual. The variable N-termini may be created by an unknown protease.<ref name='LIB:EcoGene'/> | |||
Revision as of 00:16, 3 March 2009
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| References | Suggestions |
| Gene Name | |
|---|---|
| Product(s) |
NrdA product[1]: ribonucleoside diphosphate reductase 1, alpha subunit product, B2234 product, DnaF product |
| Description |
Component of B1 protein[2],ribonucleotide reductase I[2] |
| Knock-Out Phenotype | |
| Regulation/Expression | |
| Regulation/Activity |
|
| edit table |
See Help:Quickview for help with entering information in the Quickview table.
Notes
Reducing equivalents provided by glutaredoxin or thioredoxin. Binds TrxA (Kumar, 2004). About half of the overproduced protein has the first one or two amino acids removed. E. coli B native protein is reported to start with Gln. a second native E. coli B protein start may correspond to a cleavage after Leu26. Leu26 CTG codon is unlikely to be an alternative start codon in K-12 or B and no evidence of this cleavage or an alternative start codon was seen with the overproduced K-12 protein. An M-N or MN-Q cleavage by MAP would be unusual. The variable N-termini may be created by an unknown protease.[4]
References
- ↑ 1.0 1.1 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
- ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.