Phage MS2: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
|||
| Line 1: | Line 1: | ||
Bacteriophage MS2 | Bacteriophage MS2 | ||
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nuccore&id=176120924 NC_001417] | |||
== Morphology == | == Morphology == | ||
| Line 14: | Line 14: | ||
==== RNA replication ==== | ==== RNA replication ==== | ||
* Requires the MS2-encoded protein, Replicase, as well as 3 host proteins: EF-Ts, EF-Tu & ribosomal protein S1 </ | * Requires the MS2-encoded protein, Replicase, as well as 3 host proteins: EF-Ts, EF-Tu & ribosomal protein S1 <ref name='PMID:4573038'/><ref name='PMID:4592692'/> | ||
** Produces (-) strands that are complementary to the packaged strand and (+) strands, which serve as the template mRNA for translation of the MS2 proteins & is also packaged <ref name='PMID:17775172'/><ref name='PMID:5969764 '/> | ** Produces (-) strands that are complementary to the packaged strand and (+) strands, which serve as the template mRNA for translation of the MS2 proteins & is also packaged <ref name='PMID:17775172'/><ref name='PMID:5969764 '/> | ||
** Replicase is extremely error-prone relative to DNA replication machinery | ** Replicase is extremely error-prone relative to DNA replication machinery | ||
Revision as of 19:34, 21 November 2008
Bacteriophage MS2 NC_001417
Morphology
Genome Organization
3569 bases of ssRNA, linear, (+) strand
4 genes encoded; gene order: A (maturation), coat, lysis (overlaps both coat and rep genes), rep (replicase)
Life cycle
Adsorption and Injection
Absorbs to F pilus of E. coli [1]
RNA replication
- Requires the MS2-encoded protein, Replicase, as well as 3 host proteins: EF-Ts, EF-Tu & ribosomal protein S1 [2][3]
- MS2 does NOT require Hfq, unlike Qbeta
Packaging
- T=3 morphology, with 180 copies of coat and addition of a single copy of A protein
- Packaging of RNA genome is proposed to begin with aggregation of coat protein at specific regions of the genome
Lysis
See also
References
See Help:References for how to manage references in EcoliWiki.
- ↑ LOEB, T (1960) Isolation of a bacteriophage specific for the F plus and Hfr mating types of Escherichia coli K-12. Science 131 932-3 PubMed
- ↑ Fedoroff, NV & Zinder, ND (1973) Factor requirement of the bacteriophage f2 replicase. Nature New Biol. 241 105-8 PubMed
- ↑ Lupker, JH et al. (1974) An Escherichia coli mutant with an altered elongation factor Tu. Proc. Natl. Acad. Sci. U.S.A. 71 460-3 PubMed
- ↑ Lodish, HF & Zinder, ND (1966) Replication of the RNA of Bacteriophage f2. Science 152 372-7 PubMed
- ↑ Lodish, HF & Zinder, ND (1966) Semi-conservative replication of bacteriophage f2 RNA. J. Mol. Biol. 21 207-9 PubMed
- ↑ Atkins, JF et al. (1979) Binding of mammalian ribosomes to MS2 phage RNA reveals an overlapping gene encoding a lysis function. Cell 18 247-56 PubMed
- ↑ Model, P et al. (1979) Characterization of Op3, a lysis-defective mutant of bacteriophage f2. Cell 18 235-46 PubMed
- ↑ Beremand, MN & Blumenthal, T (1979) Overlapping genes in RNA phage: a new protein implicated in lysis. Cell 18 257-66 PubMed