Phage MS2: Difference between revisions
Jump to navigation
Jump to search
| Line 15: | Line 15: | ||
==== RNA replication ==== | ==== RNA replication ==== | ||
* Requires the MS2-encoded protein, Replicase, as well as 3 host proteins: EF-Ts, EF-Tu & ribosomal protein S1 </br> | * Requires the MS2-encoded protein, Replicase, as well as 3 host proteins: EF-Ts, EF-Tu & ribosomal protein S1 </br> | ||
** Produces (-) strands that are complementary to the packaged strand and (+) strands, which serve as the template mRNA for translation of the MS2 proteins & is also packaged <ref name='PMID:5969764 '/> | ** Produces (-) strands that are complementary to the packaged strand and (+) strands, which serve as the template mRNA for translation of the MS2 proteins & is also packaged <ref name='PMID:17775172'/><ref name='PMID:5969764 '/> | ||
** Replicase is extremely error-prone relative to DNA replication machinery | ** Replicase is extremely error-prone relative to DNA replication machinery | ||
* MS2 does NOT require Hfq, unlike Qbeta | * MS2 does NOT require Hfq, unlike Qbeta | ||
Revision as of 19:27, 21 November 2008
Bacteriophage MS2 [NC_001417]
Morphology
Genome Organization
3569 bases of ssRNA, linear, (+) strand
4 genes encoded; gene order: A (maturation), coat, lysis (overlaps both coat and rep genes), rep (replicase)
Life cycle
Adsorption and Injection
Absorbs to F pilus of E. coli [1]
RNA replication
- Requires the MS2-encoded protein, Replicase, as well as 3 host proteins: EF-Ts, EF-Tu & ribosomal protein S1
- MS2 does NOT require Hfq, unlike Qbeta
Packaging
- T=3 morphology, with 180 copies of coat and addition of a single copy of A protein
- Packaging of RNA genome is proposed to begin with aggregation of coat protein at specific regions of the genome
Lysis
See also
References
See Help:References for how to manage references in EcoliWiki.
- ↑ LOEB, T (1960) Isolation of a bacteriophage specific for the F plus and Hfr mating types of Escherichia coli K-12. Science 131 932-3 PubMed
- ↑ Lodish, HF & Zinder, ND (1966) Replication of the RNA of Bacteriophage f2. Science 152 372-7 PubMed
- ↑ Lodish, HF & Zinder, ND (1966) Semi-conservative replication of bacteriophage f2 RNA. J. Mol. Biol. 21 207-9 PubMed
- ↑ Atkins, JF et al. (1979) Binding of mammalian ribosomes to MS2 phage RNA reveals an overlapping gene encoding a lysis function. Cell 18 247-56 PubMed
- ↑ Model, P et al. (1979) Characterization of Op3, a lysis-defective mutant of bacteriophage f2. Cell 18 235-46 PubMed
- ↑ Beremand, MN & Blumenthal, T (1979) Overlapping genes in RNA phage: a new protein implicated in lysis. Cell 18 257-66 PubMed