metB:Gene Product(s): Difference between revisions

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!{{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
|
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MetB product  
MetB product  
|-
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!{{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
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cystathionine gamma-synthase, PLP-dependent product<ref name='LIB:Riley_2006'/>, B3939 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MetB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
cystathionine gamma-synthase, PLP-dependent product<ref name='LIB:Riley_2006'/>, B3939 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, MetB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
|-
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!{{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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MetB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
MetB<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  


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|-
|-
!{{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
|
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** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.48 2.5.1.48]<ref name='LIB:Riley_2006'/>
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.48 2.5.1.48]<ref name='LIB:Riley_2006'/>
|-
 
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|- {{table_heading_style}}
|- {{table_heading_style}}
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
|-
|
|
[[Category:GO:0003824_!_catalytic_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0003824_!_catalytic_activity GO:0003824]
|
catalytic activity
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015421
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
|
[[Category:GO:0003824_!_catalytic_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0003824_!_catalytic_activity GO:0003824]
|
catalytic activity
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015422
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
|
[[Category:GO:0003962_!_cystathionine_gamma-synthase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0003962_!_cystathionine_gamma-synthase_activity GO:0003962]
|
cystathionine gamma-synthase activity
|
GO_REF:0000003
|
IEA: Inferred from Electronic Annotation
|
EC:2.5.1.48
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-  
|-  
|
|
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IPI: Inferred from Physical Interaction
IPI: Inferred from Physical Interaction
|
|
UniProtKB:P18843
[http://www.ebi.uniprot.org/entry/P18843 UniProtKB:P18843]
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
[[Category:GO:0005737_!_cytoplasm]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0005737_!_cytoplasm GO:0005737]
|
cytoplasm
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0963
|
C
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
[[Category:GO:0006520_!_amino_acid_metabolic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0006520_!_amino_acid_metabolic_process GO:0006520]
|
amino acid metabolic process
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000277
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
[[Category:GO:0008652_!_amino_acid_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0008652_!_amino_acid_biosynthetic_process GO:0008652]
|
amino acid biosynthetic process
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0028
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
[[Category:GO:0009086_!_methionine_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0009086_!_methionine_biosynthetic_process GO:0009086]
|
methionine biosynthetic process
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GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0486
|
P
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
[[Category:GO:0016740_!_transferase_activity]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016740_!_transferase_activity GO:0016740]
|
transferase activity
|
GO_REF:0000004
|
IEA: Inferred from Electronic Annotation
|
SP_KW:KW-0808
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
[[Category:GO:0030170_!_pyridoxal_phosphate_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0030170_!_pyridoxal_phosphate_binding GO:0030170]
|
pyridoxal phosphate binding
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR000277
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete
|-
|
 
|
[[Category:GO:0030170_!_pyridoxal_phosphate_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0030170_!_pyridoxal_phosphate_binding GO:0030170]
|
pyridoxal phosphate binding
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015421
|
|
F
F
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|
|
required fields missing   
required fields missing   
|-
|
|
[[Category:GO:0030170_!_pyridoxal_phosphate_binding]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0030170_!_pyridoxal_phosphate_binding GO:0030170]
|
pyridoxal phosphate binding
|
GO_REF:0000002
|
IEA: Inferred from Electronic Annotation
|
InterPro:IPR015422
|
F
|
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
|
complete


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Protein
Protein
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could be indirect   
could be indirect   
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!Compartment!!Description!!Evidence!!Notes
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!{{table heading style}} |Sequence
!align=left {{table heading style}} |Sequence
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||
[http://biocyc.org/ECOLI/sequence-aa?object=O-SUCCHOMOSERLYASE-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=O-SUCCHOMOSERLYASE-MONOMER at EcoCyc]  
<pre>MTRKQATIAV RSGLNDDEQY GCVVPPIHLS STYNFTGFNE PRAHDYSRRG NPTRDVVQRA
<pre>MTRKQATIAV RSGLNDDEQY GCVVPPIHLS STYNFTGFNE PRAHDYSRRG NPTRDVVQRA
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RISTGIEDGE DLIADLENGF RAANKG</pre>  
RISTGIEDGE DLIADLENGF RAANKG</pre>  
|-
|-
!{{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
|
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41.55 kDa (calc) <ref name='LIB:EcoCyc10.6'/>  
41.55 kDa (calc) <ref name='LIB:EcoCyc10.6'/>  
|-
|-
!{{table heading style}} |pI
!align=left {{table heading style}} |pI
|
||
6.43<ref name='LIB:EcoCyc10.6'/>  
6.43<ref name='LIB:EcoCyc10.6'/>  
|-
|-
!{{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
|
||
25900 - 26525 (calc based on 10 Y, 2 W, and 5 C residues)  
25900 - 26525 (calc based on 10 Y, 2 W, and 5 C residues)  
|-
 
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!|Resource type!!Source!!Notes/Reference
 
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
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|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=O-SUCCHOMOSERLYASE-MONOMER EcoCyc:O-SUCCHOMOSERLYASE-MONOMER]
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=O-SUCCHOMOSERLYASE-MONOMER EcoCyc:O-SUCCHOMOSERLYASE-MONOMER]
|
|
   
   
|-
|-  
|
|
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
|
|
[[uniprot:P00935|UniProt:P00935]]
[[uniprot:P00935|UniProt:P00935]]
|
|
   
   
|-
|-  
|
|
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
|
|
[http://pfam.sanger.ac.uk/family?acc=PF01053 PFAM:PF01053]
[http://pfam.sanger.ac.uk/family?acc=PF01053 PFAM:PF01053]
|
|
   
   
|-
|-  
|
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[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])  
|
|
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418374 RefSeq:NP_418374]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_418374 RefSeq:NP_418374]
|
|
   
   
|-
|-  
|
|
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])  
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])  
|
|
[http://salilab.org/modbase/searchbyid?databaseID=P00935 ModBase:P00935]
[http://salilab.org/modbase/searchbyid?databaseID=P00935 ModBase:P00935]
|
|
   
   
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Revision as of 02:15, 15 November 2008

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

Standard name

MetB product

Synonyms

cystathionine gamma-synthase, PLP-dependent product[1], B3939 product[2][1], MetB product[2][1]

Product description

MetB[2][3];

Component of ; O-SUCCHOMOSERLYASE-CPLX[2]

EC number (for enzymes)
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See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Notes

Function

[back to top]


Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0005515

protein binding

PMID:16606699[4]

IPI: Inferred from Physical Interaction

UniProtKB:P18843

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0009063

amino acid catabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions

Partner Type Partner Notes

Protein

Subunits of O-SUCCHOMOSERLYASE-CPLX

could be indirect

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See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Notes

metB1 is a mutant brought about by X-ray mutagenesis and is most likely a deletion mutation.

The metB1 mutation causes a requirement for methionine and produces no enzymatic activity. [6]

Localization

Compartment Description Evidence Notes
edit table

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.


Notes

Structure and Physical Properties

[back to top]


Physical Properties

Sequence

at EcoCyc 

MTRKQATIAV RSGLNDDEQY GCVVPPIHLS STYNFTGFNE PRAHDYSRRG NPTRDVVQRA
LAELEGGAGA VLTNTGMSAI HLVTTVFLKP GDLLVAPHDC YGGSYRLFDS LAKRGCYRVL
FVDQGDEQAL RAALAEKPKL VLVESPSNPL LRVVDIAKIC HLAREVGAVS VVDNTFLSPA
LQNPLALGAD LVLHSCTKYL NGHSDVVAGV VIAKDPDVVT ELAWWANNIG VTGGAFDSYL
LLRGLRTLVP RMELAQRNAQ AIVKYLQTQP LVKKLYHPSL PENQGHEIAA RQQKGFGAML
SFELDGDEQT LRRFLGGLSL FTLAESLGGV ESLISHAATM THAGMAPEAR AAAGISETLL
RISTGIEDGE DLIADLENGF RAANKG
Mol. Wt

41.55 kDa (calc) [2]

pI

6.43[2]

Extinction coefficient

25900 - 26525 (calc based on 10 Y, 2 W, and 5 C residues)

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Domains/Motifs/Modification Sites

Type Residues Description Notes
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Structure

Structures

<beststructure>metB 562</beststructure>

Models

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Notes

Gene Product Resources

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Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:O-SUCCHOMOSERLYASE-MONOMER

UniProt (EcoliWiki Page)

UniProt:P00935

PFAM (EcoliWiki Page)

PFAM:PF01053

RefSeq (EcoliWiki Page)

RefSeq:NP_418374

ModBase (EcoliWiki Page)

ModBase:P00935


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Notes

Links

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Name URL Comments
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References

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  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  5. ↑ EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. ↑ Hall, BG (1989) Selection, adaptation, and bacterial operons. Genome 31 265-71 PubMed


Categories

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