ispB:Gene Product(s): Difference between revisions

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!{{table heading style}} |Standard name
!align=left {{table heading style}} |Standard name
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IspB product  
IspB product  
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!{{table heading style}} |Synonyms
!align=left {{table heading style}} |Synonyms
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octaprenyl diphosphate synthase product<ref name='LIB:Riley_2006'/>, B3187 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cel product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhbD product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, IspB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
octaprenyl diphosphate synthase product<ref name='LIB:Riley_2006'/>, B3187 product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, Cel product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, YhbD product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>, IspB product<ref name='LIB:EcoCyc10.6'/><ref name='LIB:Riley_2006'/>  
|-
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!{{table heading style}} |Product description
!align=left {{table heading style}} |Product description
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OPPSYN-MONOMER<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  
OPPSYN-MONOMER<ref name='LIB:EcoCyc10.6'/><ref name='LIB:EcoCyc11.1'/>;  


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|-
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!{{table heading style}} |EC number (for enzymes)
!align=left {{table heading style}} |EC number (for enzymes)
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** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.- 2.5.1.-]<ref name='LIB:Riley_2006'/>
** [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.- 2.5.1.-]<ref name='LIB:Riley_2006'/>
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IPI: Inferred from Physical Interaction
IPI: Inferred from Physical Interaction
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UniProtKB:P60422
[http://www.ebi.uniprot.org/entry/P60422 UniProtKB:P60422]
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F
F
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
|-
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[[Category:GO:0016094_!_polyprenol_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016094_!_polyprenol_biosynthetic_process GO:0016094]
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polyprenol biosynthetic process
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[[PMID:11108713]]<ref name=PMID:11108713/>
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IMP: Inferred from Mutant Phenotype
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P
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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required fields missing   
required fields missing   
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[[Category:GO:0016094_!_polyprenol_biosynthetic_process]][http://gowiki.tamu.edu/wiki/index.php/Category:GO:0016094_!_polyprenol_biosynthetic_process GO:0016094]
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polyprenol biosynthetic process
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[[PMID:11108713]]<ref name=PMID:11108713/>
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IMP: Inferred from Mutant Phenotype
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P
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete


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Protein
Protein
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could be indirect   
could be indirect   
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!{{table heading style}} |Sequence
!align=left {{table heading style}} |Sequence
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[http://biocyc.org/ECOLI/sequence-aa?object=OPPSYN-MONOMER at EcoCyc]  
[http://biocyc.org/ECOLI/sequence-aa?object=OPPSYN-MONOMER at EcoCyc]  
<pre>MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM IAVLAARAVG
<pre>MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM IAVLAARAVG
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VLPDTPWREA LIGLAHIAVQ RDR</pre>  
VLPDTPWREA LIGLAHIAVQ RDR</pre>  
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!{{table heading style}} |Mol. Wt
!align=left {{table heading style}} |Mol. Wt
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35.22 kDa (calc) <ref name='LIB:EcoCyc10.6'/>  
35.22 kDa (calc) <ref name='LIB:EcoCyc10.6'/>  
|-
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!{{table heading style}} |pI
!align=left {{table heading style}} |pI
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|-
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!{{table heading style}} |Extinction coefficient
!align=left {{table heading style}} |Extinction coefficient
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24410 - 24785 (calc based on 9 Y, 2 W, and 3 C residues)  
24410 - 24785 (calc based on 9 Y, 2 W, and 3 C residues)  
|-
 
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=OPPSYN-MONOMER EcoCyc:OPPSYN-MONOMER]
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=OPPSYN-MONOMER EcoCyc:OPPSYN-MONOMER]
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[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
[http://uniprot.org/ UniProt] ([[UniProt|EcoliWiki Page]])  
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[[uniprot:P0AD57|UniProt:P0AD57]]
[[uniprot:P0AD57|UniProt:P0AD57]]
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[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
[http://pfam.sanger.ac.uk/ PFAM] ([[PFAM|EcoliWiki Page]])  
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[http://pfam.sanger.ac.uk/family?acc=PF00348 PFAM:PF00348]
[http://pfam.sanger.ac.uk/family?acc=PF00348 PFAM:PF00348]
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[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/RefSeq/ RefSeq] ([[RefSeq|EcoliWiki Page]])  
|
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[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417654 RefSeq:NP_417654]
[http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=NP_417654 RefSeq:NP_417654]
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[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])  
[http://modbase.compbio.ucsf.edu ModBase] ([[ModBase|EcoliWiki Page]])  
|
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[http://salilab.org/modbase/searchbyid?databaseID=P0AD57 ModBase:P0AD57]
[http://salilab.org/modbase/searchbyid?databaseID=P0AD57 ModBase:P0AD57]
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Revision as of 02:06, 15 November 2008

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

Standard name

IspB product

Synonyms

octaprenyl diphosphate synthase product[1], B3187 product[2][1], Cel product[2][1], YhbD product[2][1], IspB product[2][1]

Product description

OPPSYN-MONOMER[2][3];

Component of ; octaprenyl diphosphate synthase[3]

EC number (for enzymes)
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See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0004659

prenyltransferase activity

PMID:11825609[4]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0005515

protein binding

PMID:16606699[6]

IPI: Inferred from Physical Interaction

UniProtKB:P60422

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0016094

polyprenol biosynthetic process

PMID:11108713[7]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0005737

cytoplasm

C

Seeded from Riley et al 2006 [1].

required fields missing

GO:0006744

ubiquinone biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

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Interactions

Partner Type Partner Notes

Protein

Subunits of octaprenyl diphosphate synthase

could be indirect

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See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Notes

Localization

Compartment Description Evidence Notes
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See Help:Product_localization for how to add or edit information in this section of EcoliWiki.


Notes

Structure and Physical Properties

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Physical Properties

Sequence

at EcoCyc 

MNLEKINELT AQDMAGVNAA ILEQLNSDVQ LINQLGYYIV SGGGKRIRPM IAVLAARAVG
YEGNAHVTIA ALIEFIHTAT LLHDDVVDES DMRRGKATAN AAFGNAASVL VGDFIYTRAF
QMMTSLGSLK VLEVMSEAVN VIAEGEVLQL MNVNDPDITE ENYMRVIYSK TARLFEAAAQ
CSGILAGCTP EEEKGLQDYG RYLGTAFQLI DDLLDYNADG EQLGKNVGDD LNEGKPTLPL
LHAMHHGTPE QAQMIRTAIE QGNGRHLLEP VLEAMNACGS LEWTRQRAEE EADKAIAALQ
VLPDTPWREA LIGLAHIAVQ RDR
Mol. Wt

35.22 kDa (calc) [2]

pI
Extinction coefficient

24410 - 24785 (calc based on 9 Y, 2 W, and 3 C residues)

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See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Domains/Motifs/Modification Sites

Type Residues Description Notes
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See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Structure

Structures

<beststructure>ispB 562</beststructure>

Models

View models at:

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See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Notes

Gene Product Resources

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Resource type Source Notes/Reference
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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:OPPSYN-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AD57

PFAM (EcoliWiki Page)

PFAM:PF00348

RefSeq (EcoliWiki Page)

RefSeq:NP_417654

ModBase (EcoliWiki Page)

ModBase:P0AD57


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See Help:Product_accessions for help entering or editing information in this section of EcoliWiki.

Notes

Links

[back to top]


Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 1.5 1.6 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ Pan, JJ et al. (2002) Insight into the activation mechanism of Escherichia coli octaprenyl pyrophosphate synthase derived from pre-steady-state kinetic analysis. Biochim Biophys Acta 1594 64-73 PubMed
  5. ↑ 5.0 5.1 5.2 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. ↑ Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed
  7. ↑ Kainou, T et al. (2001) Dimer formation of octaprenyl-diphosphate synthase (IspB) is essential for chain length determination of ubiquinone. J Biol Chem 276 7876-83 PubMed


Categories

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