BJ5183: Difference between revisions
| Line 20: | Line 20: | ||
==Notes== | ==Notes== | ||
BJ5183 is a recBC sbc strain used for doing homologous recombination with linear dsDNA fragments. The strain originates with D. Hanahan, but it's construction is not well documented. The recombination properties of BJ5183 have been examined by Takahashi et al. <ref name='PMID:12559570'/>. | BJ5183 is a recBC sbc strain used for doing homologous recombination with linear dsDNA fragments. It has been used for construction of gene therapy vectors and combining HSV-1 cosmids<ref name='PMID:10471022'/>. The strain originates with D. Hanahan, but it's construction is not well documented. The recombination properties of BJ5183 have been examined by Takahashi et al. <ref name='PMID:12559570'/>. | ||
==References== | ==References== | ||
Revision as of 15:49, 20 August 2008
Alternate Names
BJ5183 is a component of Strategene's AdEasy system for construction of Adenovirus vectors.
Sources
Chromosomal Genotype
This genotype is inferred from the description in Takahashi et al. [1] as:
recB recC sbcB sbcC endA galK met thi-1 bioT hsdR rpsL(strR)
The nature of bioT is unclear, as this is not a listed synonym for any annotated E. coli gene.
Plasmids
not specified
Phage
not specified
Phenotypes
Construction
unknown parents.
Notes
BJ5183 is a recBC sbc strain used for doing homologous recombination with linear dsDNA fragments. It has been used for construction of gene therapy vectors and combining HSV-1 cosmids[2]. The strain originates with D. Hanahan, but it's construction is not well documented. The recombination properties of BJ5183 have been examined by Takahashi et al. [1].
References
See Help:References for how to manage references in EcoliWiki.
- ↑ 1.0 1.1 Takahashi, N et al. (2003) An Escherichia coli strain, BJ5183, that shows highly efficient conservative (two-progeny) DNA double-strand break repair of restriction breaks. Gene 303 89-97 PubMed
- ↑ Kong, Y et al. (1999) An efficient in vivo recombination cloning procedure for modifying and combining HSV-1 cosmids. J. Virol. Methods 80 129-36 PubMed