DH5 alpha: Difference between revisions
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* The [[endA:Gene|endA1]] mutation inactivates an intracellular endonuclease that degrades plasmid DNA in many miniprep methods<ref name='PMID:8479929'/>. | * The [[endA:Gene|endA1]] mutation inactivates an intracellular endonuclease that degrades plasmid DNA in many miniprep methods<ref name='PMID:8479929'/>. | ||
* The ''hsdR17'' mutation eliminates the restriction endonuclease of the EcoKI restriction-modification system, so DNA lacking the EcoKI methylation will not be degraded. | * The ''hsdR17'' mutation eliminates the restriction endonuclease of the EcoKI restriction-modification system, so DNA lacking the EcoKI methylation will not be degraded. | ||
* Δ''(lacZ)M15 is the [[lacZ alpha complementation|alpha acceptor]] allele needed for blue-white screening with many lacZ based vectors. | * Δ''(lacZ)""M15 is the [[lacZ alpha complementation|alpha acceptor]] allele needed for blue-white screening with many lacZ based vectors. | ||
* recA eliminates homologous recombination. This makes the strain somewhat sickly, but reduces deletion formation and plasmid multimerization<ref>citation needed</ref>. | * recA eliminates homologous recombination. This makes the strain somewhat sickly, but reduces deletion formation and plasmid multimerization<ref>citation needed</ref>. | ||
* glnV44 is the systematic name for SupE44, an [[Nonsense suppressors|amber suppressor]] | * glnV44 is the systematic name for SupE44, an [[Nonsense suppressors|amber suppressor]] | ||
Revision as of 16:09, 3 April 2008
Alternate Names
Sources
Chromosomal Genotype
fhuA2 Δ(argF-lacZ)U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17
Plasmids
Phage
Phenotypes
Construction
Notes
Transforms with high efficiency. Like many cloning strains, DH5 alpha has several features that make it useful for recombinant DNA methods.
- The endA1 mutation inactivates an intracellular endonuclease that degrades plasmid DNA in many miniprep methods[1].
- The hsdR17 mutation eliminates the restriction endonuclease of the EcoKI restriction-modification system, so DNA lacking the EcoKI methylation will not be degraded.
- Δ(lacZ)""M15 is the alpha acceptor allele needed for blue-white screening with many lacZ based vectors.
- recA eliminates homologous recombination. This makes the strain somewhat sickly, but reduces deletion formation and plasmid multimerization[2].
- glnV44 is the systematic name for SupE44, an amber suppressor
References
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