Phage PRD1: Difference between revisions

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Bacteriophage PRD1 is closely related to the other lipid-containing phages PR3, PR4, PR5, and L17.<ref name='PMID:7320707'/>
Bacteriophage PRD1 is closely related to the other lipid-containing phages PR3, PR4, PR5, and L17.<ref name='PMID:7320707'/> It is a plasmid-dependent phage that has been shown to infect a variety of gram-negative hosts including Escherichia coli.<ref name='PMID:4211861'/>


The full sequence is available from NCBI as [http://www.ncbi.nlm.nih.gov/nuccore/159192286 NCBI Reference Sequence: NC_001421.2]
The full sequence is available from NCBI as [http://www.ncbi.nlm.nih.gov/nuccore/159192286 NCBI Reference Sequence: NC_001421.2]

Revision as of 19:30, 11 June 2013

This page is currently under construction, and much of the information is currently just a placeholder- it may not apply to PRD1 at all.


Bacteriophage PRD1 is closely related to the other lipid-containing phages PR3, PR4, PR5, and L17.[1] It is a plasmid-dependent phage that has been shown to infect a variety of gram-negative hosts including Escherichia coli.[2]

The full sequence is available from NCBI as NCBI Reference Sequence: NC_001421.2


Morphology

Genome Organization

159192286 on NCBI

<gbrowseImage> name=NC_001421:1..14927 source=bacteriophage_PRD1 width=800 type=Genes </gbrowseImage>


PRD1 has a linear, 14927 bp genome of DNA.

There are 11 genes encoded on the genome; two are overlapping in-frame genes. Five of the encoded proteins make up the viron, three are needed for synthesis and the rest are for assembly. [3] The genes are generally referred to by Roman numerals I-XI as listed on the Phage f1 page. Genes are in the order II (X), V, VII, IX, VIII, III, VI, I (XI), IV, intergenic region (IG or IGR). [4] The IGR contains the packing signal (PS) as well as sequences that dictate termination, nicking for replication, and the binding of protein II and IHF. [4]

Life cycle

Adsorption and Injection

DNA replication

Packaging

Lysis

See also

References

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  1. ↑ Bamford, DH et al. (1981) Comparison of the lipid-containing bacteriophages PRD1, PR3, PR4, PR5 and L17. J. Gen. Virol. 57 365-73 PubMed
  2. ↑ Olsen, RH et al. (1974) Characteristics of PRD1, a plasmid-dependent broad host range DNA bacteriophage. J. Virol. 14 689-99 PubMed
  3. ↑ Russel, M et al. (1997) Filamentous phage assembly: variation on a protein export theme. Gene 192 23-32 PubMed
  4. ↑ 4.0 4.1 Edited by Richard Calendar (2006) The Bacteriophages (Oxford University Press, Oxford, NY) ISBN 0-19-514850-9