DNA Replication: Difference between revisions
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*Extrusion Capture Model- DNA is fed from the centrally located holoenzyme to the poles after replication<ref name='PMID:11511534'/><ref name='PMID:11169104'/> | *Extrusion Capture Model- DNA is fed from the centrally located holoenzyme to the poles after replication<ref name='PMID:11511534'/><ref name='PMID:11169104'/> | ||
*Sister Chromosome Cohesion Model- sister chromosomes are paired after replication and separated some time afterwards<ref name='PMID:10886362'/><ref name='PMID:11886555'/><ref name='PMID:15960977'/> | *Sister Chromosome Cohesion Model- sister chromosomes are paired after replication and separated some time afterwards<ref name='PMID:10886362'/><ref name='PMID:11886555'/><ref name='PMID:15960977'/> | ||
Li ''et al.'' (2002) used [[Methods:FISH|FISH]] in order to resolve the differences in these two models<ref name='PMID:12421305'/>. | Li ''et al.'' (2002) used [[Methods:Flow Cytometry| Flow Cytometry]] and [[Methods:FISH|FISH]] in order to resolve the differences in these two models<ref name='PMID:12421305'/>. | ||
==References== | ==References== | ||
Revision as of 18:29, 29 June 2010
See Help:Processes for more information about physiological processes in EcoliWiki.
General Description
Components
The catalytic core of DNA Polymerase consists of 3 proteins: xx. The fully functional holoenzyme consists of around ten separate components:
Function
Regulation
Cell Biology
Replication of the circular E.coli chromosome occurs bidirectionally from oriC.
There are several models for replication:
After replication, the daughter chromosomes must be segregated using various mechanisms.
Experimental Resources
Comparison with other organisms
Notes
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There are several models explaining how DNA segregates as it's replicated:
- Extrusion Capture Model- DNA is fed from the centrally located holoenzyme to the poles after replication[4][5]
- Sister Chromosome Cohesion Model- sister chromosomes are paired after replication and separated some time afterwards[6][7][8]
Li et al. (2002) used Flow Cytometry and FISH in order to resolve the differences in these two models[9].
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Dingman, CW (1974) Bidirectional chromosome replication: some topological considerations. J. Theor. Biol. 43 187-95 PubMed
- ↑ Lemon, KP & Grossman, AD (1998) Localization of bacterial DNA polymerase: evidence for a factory model of replication. Science 282 1516-9 PubMed
- ↑ Lemon, KP & Grossman, AD (2000) Movement of replicating DNA through a stationary replisome. Mol. Cell 6 1321-30 PubMed
- ↑ Lemon, KP & Grossman, AD (2001) The extrusion-capture model for chromosome partitioning in bacteria. Genes Dev. 15 2031-41 PubMed
- ↑ Roos, M et al. (2001) The replicated ftsQAZ and minB chromosomal regions of Escherichia coli segregate on average in line with nucleoid movement. Mol. Microbiol. 39 633-40 PubMed
- ↑ Hiraga, S et al. (2000) Bidirectional migration of SeqA-bound hemimethylated DNA clusters and pairing of oriC copies in Escherichia coli. Genes Cells 5 327-41 PubMed
- ↑ Sunako, Y et al. (2001) Sister chromosome cohesion of Escherichia coli. Mol. Microbiol. 42 1233-41 PubMed
- ↑ Bates, D & Kleckner, N (2005) Chromosome and replisome dynamics in E. coli: loss of sister cohesion triggers global chromosome movement and mediates chromosome segregation. Cell 121 899-911 PubMed
- ↑ Li, Y et al. (2002) The segregation of the Escherichia coli origin and terminus of replication. Mol. Microbiol. 46 985-96 PubMed